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    vps26-antibody-ab245575.pdf

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Signal Transduction Protein Trafficking Vesicle Transport Regulation
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Anti-VPS26 antibody (ab245575)

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Abpromise

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Western blot - Anti-VPS26 antibody (ab245575)

    Key features and details

    • Rabbit polyclonal to VPS26
    • Suitable for: WB
    • Reacts with: Mouse, Human
    • Isotype: IgG

    You may also be interested in

    Protein
    Product image
    Recombinant Human VPS26 protein (ab130047)
    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-VPS26 antibody
      See all VPS26 primary antibodies
    • Description

      Rabbit polyclonal to VPS26
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Mouse, Human
      Predicted to work with: Rat, Cow
    • Immunogen

      Synthetic peptide within Human VPS26 aa 277-327. The exact sequence is proprietary.
      Database link: O75436

    • Positive control

      • WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysate.
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7
      Preservative: 0.09% Sodium azide
      Constituent: Tris citrate/phosphate

      pH 7 to 8
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      ab245575 was affinity purified using an epitope specific to VPS26 immobilized on solid support.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Signal Transduction
      • Protein Trafficking
      • Vesicle Transport
      • Regulation
      • Signal Transduction
      • Protein Trafficking
      • Golgi Proteins

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Positive Controls

      • HeLa whole cell lysate (ab150035)
      • HeLa whole cell lysate (ab29545)
      • Jurkat whole cell lysate (ab30128)
      • NIH 3T3 whole cell lysate (ab7179)
      • Jurkat whole cell lysate (ab7899)
    • Recombinant Protein

      • Recombinant Human VPS26 protein (ab130047)
    • Related Products

      • Recombinant Human VPS26 protein (ab130047)

    Applications

    Our Abpromise guarantee covers the use of ab245575 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB 1/1000 - 1/5000. Predicted molecular weight: 38 kDa.

    Target

    • Function

      Essential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA).
    • Sequence similarities

      Belongs to the VPS26 family.
    • Cellular localization

      Cytoplasm. Endosome membrane.
    • Target information above from: UniProt accession O75436 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 510574 Cow
      • Entrez Gene: 9559 Human
      • Entrez Gene: 30930 Mouse
      • Entrez Gene: 361846 Rat
      • Omim: 605506 Human
      • SwissProt: Q0VD53 Cow
      • SwissProt: O75436 Human
      • SwissProt: P40336 Mouse
      • SwissProt: Q6AY86 Rat
      • Unigene: 499925 Human
      • Unigene: 260703 Mouse
      • Unigene: 473468 Mouse
      • Unigene: 3673 Rat
      see all
    • Alternative names

      • FLJ12930 antibody
      • HB 58 antibody
      • HB58 antibody
      • Hbeta 58 antibody
      • Hbeta 58 homolog antibody
      • Hbeta58 antibody
      • Hbeta58 homolog antibody
      • hVPS 26 antibody
      • hVPS26 antibody
      • MGC94168 antibody
      • PEP 8A antibody
      • PEP8A antibody
      • Vacuolar protein sorting 26 A antibody
      • Vacuolar protein sorting 26 antibody
      • Vacuolar protein sorting 26 homolog A antibody
      • Vacuolar protein sorting 26 homolog A S. pombe antibody
      • Vacuolar protein sorting 26 homolog A yeast antibody
      • Vacuolar protein sorting 26 yeast antibody
      • Vacuolar protein sorting 26 yeast homolog antibody
      • Vacuolar protein sorting 26A antibody
      • Vacuolar protein sorting associated protein 26A antibody
      • Vacuolar protein sorting-associated protein 26A antibody
      • Vesicle protein sorting 26 antibody
      • Vesicle protein sorting 26A antibody
      • VP26A_HUMAN antibody
      • VPS 26 antibody
      • VPS 26A antibody
      • VPS26A antibody
      see all

    Images

    • Western blot - Anti-VPS26 antibody (ab245575)
      Western blot - Anti-VPS26 antibody (ab245575)
      All lanes : Anti-VPS26 antibody (ab245575) at 0.4 µg/ml

      Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
      Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
      Lane 4 : TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate
      Lane 5 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate

      Lysates/proteins at 50 µg per lane.

      Developed using the ECL technique.

      Predicted band size: 38 kDa


      Exposure time: 10 seconds


      Prepared using NETN lysis buffer.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab245575? Please let us know so that we can cite the reference in this datasheet.

    ab245575 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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