Product nameAnti-VPS26 antibody
See all VPS26 primary antibodies
DescriptionRabbit polyclonal to VPS26
Tested applicationsSuitable for: WBmore details
Unsuitable for: IP
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow
Synthetic peptide within Human VPS26 aa 277-327. The exact sequence is proprietary.
Database link: O75436
- WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab245575 was affinity purified using an epitope specific to VPS26 immobilized on solid support.
Our Abpromise guarantee covers the use of ab245575 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 38 kDa.|
FunctionEssential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA).
Sequence similaritiesBelongs to the VPS26 family.
Cellular localizationCytoplasm. Endosome membrane.
- Information by UniProt
- FLJ12930 antibody
- HB 58 antibody
- HB58 antibody
All lanes : Anti-VPS26 antibody (ab245575) at 0.4 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 38 kDa
Exposure time: 10 seconds
Prepared using NETN lysis buffer.
ab245575 has not yet been referenced specifically in any publications.