Anti-VPS26 antibody (ab245575)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-VPS26 antibody
See all VPS26 primary antibodies -
Description
Rabbit polyclonal to VPS26 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow
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Immunogen
Synthetic peptide within Human VPS26 aa 277-327. The exact sequence is proprietary.
Database link: O75436 -
Positive control
- WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab245575 was affinity purified using an epitope specific to VPS26 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab245575 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/5000. Predicted molecular weight: 38 kDa. |
Application notes
Is unsuitable for IP.
Target
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Function
Essential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA). -
Sequence similarities
Belongs to the VPS26 family. -
Cellular localization
Cytoplasm. Endosome membrane. - Information by UniProt
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Database links
- Entrez Gene: 510574 Cow
- Entrez Gene: 9559 Human
- Entrez Gene: 30930 Mouse
- Entrez Gene: 361846 Rat
- Omim: 605506 Human
- SwissProt: Q0VD53 Cow
- SwissProt: O75436 Human
- SwissProt: P40336 Mouse
see all -
Alternative names
- FLJ12930 antibody
- HB 58 antibody
- HB58 antibody
see all
Images
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Western blot - Anti-VPS26 antibody (ab245575)All lanes : Anti-VPS26 antibody (ab245575) at 0.4 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 38 kDa
Exposure time: 10 secondsPrepared using NETN lysis buffer.
Datasheets and documents
References
ab245575 has not yet been referenced specifically in any publications.
