Anti-VPS26 antibody [CL2287] - C-terminal (ab211530)
Key features and details
- Mouse monoclonal [CL2287] to VPS26 - C-terminal
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-VPS26 antibody [CL2287] - C-terminal
See all VPS26 primary antibodies -
Description
Mouse monoclonal [CL2287] to VPS26 - C-terminal -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow -
Immunogen
Recombinant fragment corresponding to Human VPS26 aa 300 to the C-terminus (C terminal).
Database link: O75436 -
Positive control
- U251 MG cell line lysate; Human fallopian tube, small intestine, testis, and kidney tissues; HeLa , A431 , MCF7, U2OS and U251 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
CL2287 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab211530 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/200 - 1/500. Predicted molecular weight: 38 kDa.
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ICC/IF |
Use a concentration of 2 - 10 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100 |
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/200 - 1/500. Predicted molecular weight: 38 kDa. |
ICC/IF
Use a concentration of 2 - 10 µg/ml. Fixation/Permeabilization: PFA/Triton X-100 |
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Essential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA). -
Sequence similarities
Belongs to the VPS26 family. -
Cellular localization
Cytoplasm. Endosome membrane. - Information by UniProt
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Database links
- Entrez Gene: 510574 Cow
- Entrez Gene: 9559 Human
- Entrez Gene: 30930 Mouse
- Entrez Gene: 361846 Rat
- Omim: 605506 Human
- SwissProt: Q0VD53 Cow
- SwissProt: O75436 Human
- SwissProt: P40336 Mouse
see all -
Alternative names
- FLJ12930 antibody
- HB 58 antibody
- HB58 antibody
see all
Images
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All lanes : Anti-VPS26 antibody [CL2287] - C-terminal (ab211530)
Lane 1 : U-251 cells, transfected with control siRNA
Lane 2 : U-251 cells, transfected with target specific siRNA probe #1
Lane 3 : U-251 cells, transfected with target specific siRNA probe #2
Predicted band size: 38 kDaDownregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-PPIB monoclonal antibody was used as loading control.
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Immunofluorescent analysis of U251 cells (PFA-fixed/Triton X-100 permeabilized) labeling VPS26 with ab211530 at 4 μg/ml dilution (green). Microtubule- and nuclear probes are visualized in red and blue respectively.
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Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling VPS26 with ab211530 at 1/200 dilution.
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Immunofluorescent analysis of U2OS cells (PFA-fixed/Triton X-100 permeabilized) labeling VPS26 with ab211530 at 4 μg/ml dilution (green). Microtubule- and nuclear probes are visualized in red and blue respectively.
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Immunofluorescent analysis of MCF7 cells (PFA-fixed/Triton X-100 permeabilized) labeling VPS26 with ab211530 at 4 μg/ml dilution (green). Microtubule- and nuclear probes are visualized in red and blue respectively.
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Immunofluorescent analysis of A431 cells (PFA-fixed/Triton X-100 permeabilized) labeling VPS26 with ab211530 at 4 μg/ml dilution (green). Microtubule- and nuclear probes are visualized in red and blue respectively.
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Immunofluorescent analysis of HeLa cells (PFA-fixed/Triton X-100 permeabilized) labeling VPS26 with ab211530 at 4 μg/ml dilution (green). Microtubule- and nuclear probes are visualized in red and blue respectively.
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Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling VPS26 with ab211530 at 1/200 dilution.
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Immunohistochemical analysis of paraffin-embedded Human small intestine tissue labeling VPS26 with ab211530 at 1/200 dilution.
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Immunohistochemical analysis of paraffin-embedded Human fallopian tube tissue labeling VPS26 with ab211530 at 1/200 dilution.
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Anti-VPS26 antibody [CL2287] - C-terminal (ab211530) at 1/200 dilution + U251 MG cell line lysate
Predicted band size: 38 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab211530 has not yet been referenced specifically in any publications.