Overview

  • Product name

    Anti-VPS26 antibody [CL2287] - C-terminal
    See all VPS26 primary antibodies
  • Description

    Mouse monoclonal [CL2287] to VPS26 - C-terminal
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment corresponding to Human VPS26 aa 302-327 (C terminal).
    Sequence:

    LRKQRTNFHQRFESPESQASAEQPEM


    Database link: O75436

  • Positive control

    • U251 MG cell line lysate; Human fallopian tube, small intestine, testis, and kidney tissues; HeLa , A431 , MCF7, U2OS and U251 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab211530 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200 - 1/500. Predicted molecular weight: 38 kDa.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Fixation/Permeabilization: PFA/Triton X-100

IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Essential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA).
  • Sequence similarities

    Belongs to the VPS26 family.
  • Cellular localization

    Cytoplasm. Endosome membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ12930 antibody
    • HB 58 antibody
    • HB58 antibody
    • Hbeta 58 antibody
    • Hbeta 58 homolog antibody
    • Hbeta58 antibody
    • Hbeta58 homolog antibody
    • hVPS 26 antibody
    • hVPS26 antibody
    • MGC94168 antibody
    • PEP 8A antibody
    • PEP8A antibody
    • Vacuolar protein sorting 26 A antibody
    • Vacuolar protein sorting 26 antibody
    • Vacuolar protein sorting 26 homolog A antibody
    • Vacuolar protein sorting 26 homolog A S. pombe antibody
    • Vacuolar protein sorting 26 homolog A yeast antibody
    • Vacuolar protein sorting 26 yeast antibody
    • Vacuolar protein sorting 26 yeast homolog antibody
    • Vacuolar protein sorting 26A antibody
    • Vacuolar protein sorting associated protein 26A antibody
    • Vacuolar protein sorting-associated protein 26A antibody
    • Vesicle protein sorting 26 antibody
    • Vesicle protein sorting 26A antibody
    • VP26A_HUMAN antibody
    • VPS 26 antibody
    • VPS 26A antibody
    • VPS26A antibody
    see all

Images

  • All lanes : Anti-VPS26 antibody [CL2287] - C-terminal (ab211530)

    Lane 1 : U-251 cells, transfected with control siRNA
    Lane 2 : U-251 cells, transfected with target specific siRNA probe #1
    Lane 3 : U-251 cells, transfected with target specific siRNA probe #2

    Predicted band size: 38 kDa



    Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-PPIB monoclonal antibody was used as loading control.

  • Immunofluorescent analysis of U251 cells (PFA-fixed/Triton X-100 permeabilized) labeling VPS26 with ab211530 at 4 μg/ml dilution (green). Microtubule- and nuclear probes are visualized in red and blue respectively.

  • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling VPS26 with ab211530 at 1/200 dilution.

  • Immunofluorescent analysis of U2OS cells (PFA-fixed/Triton X-100 permeabilized) labeling VPS26 with ab211530 at 4 μg/ml dilution (green). Microtubule- and nuclear probes are visualized in red and blue respectively.

  • Immunofluorescent analysis of MCF7 cells (PFA-fixed/Triton X-100 permeabilized) labeling VPS26 with ab211530 at 4 μg/ml dilution (green). Microtubule- and nuclear probes are visualized in red and blue respectively.

  • Immunofluorescent analysis of A431 cells (PFA-fixed/Triton X-100 permeabilized) labeling VPS26 with ab211530 at 4 μg/ml dilution (green). Microtubule- and nuclear probes are visualized in red and blue respectively.

  • Immunofluorescent analysis of HeLa cells (PFA-fixed/Triton X-100 permeabilized) labeling VPS26 with ab211530 at 4 μg/ml dilution (green). Microtubule- and nuclear probes are visualized in red and blue respectively.

  • Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling VPS26 with ab211530 at 1/200 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human small intestine tissue labeling VPS26 with ab211530 at 1/200 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human fallopian tube tissue labeling VPS26 with ab211530 at 1/200 dilution.

  • Anti-VPS26 antibody [CL2287] - C-terminal (ab211530) at 1/200 dilution + U251 MG cell line lysate

    Predicted band size: 38 kDa

References

ab211530 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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