Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13455(B)] to VPS26 - N-terminal
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Product nameAnti-VPS26 antibody [EPR13455(B)] - N-terminal
See all VPS26 primary antibodies
DescriptionRabbit monoclonal [EPR13455(B)] to VPS26 - N-terminal
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human VPS26 aa 1-100 (N terminal) (Cysteine residue). The exact sequence is proprietary.
Database link: O75436
- WB: HepG2, HeLa and 293T cell lysates.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab180172 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 38 kDa.|
FunctionEssential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA).
Sequence similaritiesBelongs to the VPS26 family.
Cellular localizationCytoplasm. Endosome membrane.
- Information by UniProt
- FLJ12930 antibody
- HB 58 antibody
- HB58 antibody
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: VPS26 knockout HAP1 cell lysate (20 µg)
Lane 3: Jurkat cell lysate (20 µg)
Lane 4: K562 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab180172 observed at 40 kDa. Red - loading control, ab18058, observed at 124 kDa.
ab180172 was shown to specifically react with VPS26 when VPS26 knockout samples were used. Wild-type and VPS26 knockout samples were subjected to SDS-PAGE. ab180172 and ab18058 (loading control to Vinculin) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10 000 dilution for 1 hour at room temperature before imaging.
All lanes : Anti-VPS26 antibody [EPR13455(B)] - N-terminal (ab180172) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Standard HRP labeled goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 38 kDa
ab180172 has not yet been referenced specifically in any publications.