Product nameAnti-VPS26 antibody [EPR13456]
See all VPS26 primary antibodies
DescriptionRabbit monoclonal [EPR13456] to VPS26
Tested applicationsSuitable for: WB, ICC/IF, IPmore details
Unsuitable for: Flow Cyt or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human VPS26 aa 250 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: O75436
- HepG2, HeLa, 293T, and A431 cell lysates; HeLa cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab181352 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 38 kDa.|
|ICC/IF||1/100 - 1/250.|
|IP||1/10 - 1/100.|
FunctionEssential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA).
Sequence similaritiesBelongs to the VPS26 family.
Cellular localizationCytoplasm. Endosome membrane.
- Information by UniProt
- FLJ12930 antibody
- HB 58 antibody
- HB58 antibody
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: VPS26 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: HepG2 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab181352 observed at 40 kDa. Red - loading control, ab18058, observed at 124 kDa.
ab181352 was shown to specifically react with VPS26 when VPS26 knockout samples were used. Wild-type and VPS26 knockout samples were subjected to SDS-PAGE. ab181352 and ab18058 (loading control to Vinculin) were diluted to 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ( ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Immunofluorescent analysis of HeLa cells labeling VPS26 with ab181352 at 1/100 dilution (red). DAPI nuclear staining (blue).
All lanes : Anti-VPS26 antibody [EPR13456] (ab181352) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293T cell lysate
Lane 4 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 38 kDa
Western blot analysis on immunoprecipitation pellet from HeLa cell lysate pulling down VPS26 using ab181352 at 1/10 dilution.
This product has been referenced in:
- Sapmaz A et al. USP32 regulates late endosomal transport and recycling through deubiquitylation of Rab7. Nat Commun 10:1454 (2019). Read more (PubMed: 30926795) »
- Mir R et al. The Parkinson's disease VPS35[D620N] mutation enhances LRRK2-mediated Rab protein phosphorylation in mouse and human. Biochem J 475:1861-1883 (2018). Read more (PubMed: 29743203) »