Key features and details
- Rabbit polyclonal to VPS26B
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-VPS26B antibody
DescriptionRabbit polyclonal to VPS26B
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Xenopus laevis, Orangutan, Xenopus tropicalis
Recombinant full length protein corresponding to Human VPS26B aa 1-336.
MSFFGFGQSVEVEILLNDAESRKRAEHKTEDGKKEKYFLFYDGETVSGKV SLALKNPNKRLEHQGIKIEFIGQIELYYDRGNHHEFVSLVKDLARPGEIT QSQAFDFEFTHVEKPYESYTGQNVKLRYFLRATISRRLNDVVKEMDIVVH TLSTYPELNSSIKMEVGIEDCLHIEFEYNKSKYHLKDVIVGKIYFLLVRI KIKHMEIDIIKRETTGTGPNVYHENDTIAKYEIMDGAPVRGESIPIRLFL AGYELTPTMRDINKKFSVRYYLNLVLIDEEERRYFKQQEVVLWRKGDIVR KSMSHQAAIASQRFEGTTSLGEVRTPSQLSDNNCRQ
Database link: Q4G0F5
- WB: Mouse brain tissue lysate. IHC-P: Human colon cancer tissue. ICC/IF: PC-3 cells.
This product was previously labelled as VP26B.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: PBS, 50% Glycerol, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%
Our Abpromise guarantee covers the use of ab236966 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000.|
|IHC-P||1/20 - 1/200.|
|ICC/IF||1/50 - 1/200.|
FunctionActs as component of the retromer cargo-selective complex (CSC). The CSC is believed to be the core functional component of retromer or respective retromer complex variants acting to prevent missorting of selected transmembrane cargo proteins into the lysosomal degradation pathway. The recruitment of the CSC to the endosomal membrane involves RAB7A and SNX3. The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN) and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX3-retromer mediates the retrograde transport of WLS distinct from the SNX-BAR retromer pathway. The SNX27-retromer is believed to be involved in endosome-to-plasma membrane trafficking and recycling of a broad spectrum of cargo proteins. The CSC seems to act as recruitment hub for other proteins, such as the WASH complex and TBC1D5. May be involved in retrograde transport of SORT1 but not of IGF2R. Acts redundantly with VSP26A in SNX-27 mediated endocytic recycling of SLC2A1/GLUT1.
Sequence similaritiesBelongs to the VPS26 family.
Cellular localizationCytoplasm. Membrane. Early endosome. Late endosome. Localizes to early and late endosomal structures (By similarity).
- Information by UniProt
- Pep8b antibody
- Vacuolar protein sorting 26 homolog B (S. pombe) antibody
- Vacuolar protein sorting 26 homolog B (yeast) antibody
Anti-VPS26B antibody (ab236966) at 1/500 dilution + Mouse brain tissue lysate
Goat polyclonal to rabbit IgG at 1/50000 dilution
Paraffin-embedded human colon cancer tissue stained for VPS26B using ab236966 at 1/100 dilution in immunohistochemical analysis.
PC-3 (human prostate adenocarcinoma cell line) cells stained for VPS26B (Green) using ab236966 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236966 has not yet been referenced specifically in any publications.