Anti-VPS28 antibody [EPR9880] (ab154793)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-VPS28 antibody [EPR9880]
See all VPS28 primary antibodies -
DescriptionRabbit monoclonal [EPR9880] to VPS28
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: IHC-P -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide within Human VPS28. The exact sequence is proprietary.
Database link: Q9UK41 -
Positive control
- WB: HepG2, Jurkat and LnCaP cell lysates and mouse and rat brain tissue lysates. IP: Jurkat cell lysate.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberEPR9880
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IsotypeIgG
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Research areas
Associated products
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Isotype control
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Positive Controls
- Mouse brain tissue lysate - total protein (ab30151)
- Rat brain cerebellum tissue lysate - total protein (ab4032)
- Mouse brain tissue lysate - total protein (0 days) (ab7188)
- Mouse brain tissue lysate - total protein (14 days) (ab7189)
- Mouse brain tissue lysate - total protein (7 days) (ab7190)
- Jurkat whole cell lysate (ab7899)
- HepG2 whole cell lysate (ab7900)
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Related Products
Applications
Our Abpromise guarantee covers the use of ab154793 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 25 kDa. | |
| IP | 1/50. |
Target
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FunctionComponent of the ESCRT-I complex, a regulator of vesicular trafficking process.
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Sequence similaritiesBelongs to the VPS28 family.
Contains 1 VPS28 C-terminal domain.
Contains 1 VPS28 N-terminal domain. -
Cellular localizationCell membrane. Late endosome membrane.
- Information by UniProt
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Database links
- Entrez Gene: 51160 Human
- Entrez Gene: 66914 Mouse
- Entrez Gene: 300052 Rat
- Omim: 611952 Human
- SwissProt: Q9UK41 Human
- SwissProt: Q9D1C8 Mouse
- SwissProt: B5DEN9 Rat
- Unigene: 418175 Human
see all -
Alternative names
- ESCRT-I complex subunit VPS28 antibody
- H-Vps28 antibody
- Vacuolar protein sorting 28 antibody
see all
Images
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![Western blot - Anti-VPS28 antibody [EPR9880] (ab154793)](https://www.abcam.com/ps/products/154/ab154793/Images/ab154793-240899-ab154793upwb.jpg)
Western blot - Anti-VPS28 antibody [EPR9880] (ab154793)Anti-VPS28 antibody [EPR9880] (ab154793) at 1/2000 dilution (unpurified) + HepG2 cell lysate at 10 µg
Secondary
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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![Western blot - Anti-VPS28 antibody [EPR9880] (ab154793)](https://www.abcam.com/ps/products/154/ab154793/Images/ab154793-240900-ab154793pwb.jpg)
Western blot - Anti-VPS28 antibody [EPR9880] (ab154793)Anti-VPS28 antibody [EPR9880] (ab154793) at 1/1500 dilution (purified) + HepG2 cell lysate at 10 µg
Secondary
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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![Western blot - Anti-VPS28 antibody [EPR9880] (ab154793)](https://www.abcam.com/ps/products/154/ab154793/Images/ab154793-240901-ab154793upwb2.jpg)
Western blot - Anti-VPS28 antibody [EPR9880] (ab154793)All lanes : Anti-VPS28 antibody [EPR9880] (ab154793) at 1/2000 dilution (unpurified)
Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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![Western blot - Anti-VPS28 antibody [EPR9880] (ab154793)](https://www.abcam.com/ps/products/154/ab154793/Images/ab154793-240902-ab154793pwb2.jpg)
Western blot - Anti-VPS28 antibody [EPR9880] (ab154793)All lanes : Anti-VPS28 antibody [EPR9880] (ab154793) at 1/1500 dilution (purified)
Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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![Western blot - Anti-VPS28 antibody [EPR9880] (ab154793)](https://www.abcam.com/ps/datasheet/images/154/ab154793/VPS28-Primary-antibodies-ab154793-1.JPG)
Western blot - Anti-VPS28 antibody [EPR9880] (ab154793)All lanes : Anti-VPS28 antibody [EPR9880] (ab154793) at 1/1000 dilution (unpurified)
Lane 1 : HepG2 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : LnCaP cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 25 kDa -
![Immunoprecipitation - Anti-VPS28 antibody [EPR9880] (ab154793)](https://www.abcam.com/ps/products/154/ab154793/Images/ab154793-240903-ab154793upip.jpg)
Immunoprecipitation - Anti-VPS28 antibody [EPR9880] (ab154793)ab154793 (unpurified) at 1/50 immunoprecipitating VPS28 in jurkat cell lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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![Immunoprecipitation - Anti-VPS28 antibody [EPR9880] (ab154793)](https://www.abcam.com/ps/products/154/ab154793/Images/ab154793-240904-ab154793pip.jpg)
Immunoprecipitation - Anti-VPS28 antibody [EPR9880] (ab154793)ab154793 (purified) at 1/50 immunoprecipitating VPS28 in jurkat cell lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Datasheets and documents
References
ab154793 has not yet been referenced specifically in any publications.
