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RecombinantRabMAb

Recombinant Anti-VPS28 antibody [EPR9880] (ab154793)

  • Datasheet
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Western blot - Anti-VPS28 antibody [EPR9880] (ab154793)
  • Western blot - Anti-VPS28 antibody [EPR9880] (ab154793)
  • Western blot - Anti-VPS28 antibody [EPR9880] (ab154793)
  • Western blot - Anti-VPS28 antibody [EPR9880] (ab154793)
  • Western blot - Anti-VPS28 antibody [EPR9880] (ab154793)
  • Immunoprecipitation - Anti-VPS28 antibody [EPR9880] (ab154793)
  • Immunoprecipitation - Anti-VPS28 antibody [EPR9880] (ab154793)
  • Anti-VPS28 antibody [EPR9880] (ab154793)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9880] to VPS28
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Protein
Product image
Recombinant Human VPS28 protein (ab87718)

View more associated products

Overview

  • Product name

    Anti-VPS28 antibody [EPR9880]
    See all VPS28 primary antibodies
  • Description

    Rabbit monoclonal [EPR9880] to VPS28
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Mouse
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human VPS28. The exact sequence is proprietary.
    Database link: Q9UK41

  • Positive control

    • WB: HepG2, Jurkat and LnCaP cell lysates and mouse and rat brain tissue lysates. IP: Jurkat cell lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR9880
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Vacuole
    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Regulation

Associated products

  • Alternative Versions

    • Anti-VPS28 antibody [EPR9880] - BSA and Azide free (ab249102)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Mouse brain tissue lysate - total protein (ab30151)
    • Rat brain cerebellum tissue lysate - total protein (ab4032)
    • Mouse brain tissue lysate - total protein (0 days) (ab7188)
    • Mouse brain tissue lysate - total protein (14 days) (ab7189)
    • Mouse brain tissue lysate - total protein (7 days) (ab7190)
    • Jurkat whole cell lysate (ab7899)
  • Recombinant Protein

    • Recombinant Human VPS28 protein (ab87718)
  • Related Products

    • Recombinant Human VPS28 protein (ab87718)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab154793 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IP
Human
WB
Mouse
Human
Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 25 kDa.
IP
1/50.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 25 kDa.
IP
1/50.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function

      Component of the ESCRT-I complex, a regulator of vesicular trafficking process.
    • Sequence similarities

      Belongs to the VPS28 family.
      Contains 1 VPS28 C-terminal domain.
      Contains 1 VPS28 N-terminal domain.
    • Cellular localization

      Cell membrane. Late endosome membrane.
    • Target information above from: UniProt accession Q9UK41 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 51160 Human
      • Entrez Gene: 66914 Mouse
      • Entrez Gene: 300052 Rat
      • Omim: 611952 Human
      • SwissProt: Q9UK41 Human
      • SwissProt: Q9D1C8 Mouse
      • SwissProt: B5DEN9 Rat
      • Unigene: 418175 Human
      • Unigene: 30028 Mouse
      • Unigene: 36912 Rat
      see all
    • Alternative names

      • ESCRT-I complex subunit VPS28 antibody
      • H-Vps28 antibody
      • Vacuolar protein sorting 28 antibody
      • Vacuolar protein sorting 28 (yeast) antibody
      • Vacuolar protein sorting 28 homolog (S. cerevisiae) antibody
      • Vacuolar protein sorting 28 homolog antibody
      • Vacuolar protein sorting-associated protein 28 homolog antibody
      • VPS 28 antibody
      • Vps28 antibody
      • VPS28_HUMAN antibody
      • Yeast class E protein Vps28p homolog antibody
      see all

    Images

    • Western blot - Anti-VPS28 antibody [EPR9880] (ab154793)
      Western blot - Anti-VPS28 antibody [EPR9880] (ab154793)
      Anti-VPS28 antibody [EPR9880] (ab154793) at 1/2000 dilution (unpurified) + HepG2 cell lysate at 10 µg

      Secondary
      Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size: 25 kDa
      Observed band size: 25 kDa



      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Western blot - Anti-VPS28 antibody [EPR9880] (ab154793)
      Western blot - Anti-VPS28 antibody [EPR9880] (ab154793)
      Anti-VPS28 antibody [EPR9880] (ab154793) at 1/1500 dilution (purified) + HepG2 cell lysate at 10 µg

      Secondary
      Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size: 25 kDa
      Observed band size: 25 kDa



      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Western blot - Anti-VPS28 antibody [EPR9880] (ab154793)
      Western blot - Anti-VPS28 antibody [EPR9880] (ab154793)
      All lanes : Anti-VPS28 antibody [EPR9880] (ab154793) at 1/2000 dilution (unpurified)

      Lane 1 : Mouse brain tissue lysate
      Lane 2 : Rat brain tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size: 25 kDa
      Observed band size: 25 kDa



      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Western blot - Anti-VPS28 antibody [EPR9880] (ab154793)
      Western blot - Anti-VPS28 antibody [EPR9880] (ab154793)
      All lanes : Anti-VPS28 antibody [EPR9880] (ab154793) at 1/1500 dilution (purified)

      Lane 1 : Mouse brain tissue lysate
      Lane 2 : Rat brain tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size: 25 kDa
      Observed band size: 25 kDa



      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Western blot - Anti-VPS28 antibody [EPR9880] (ab154793)
      Western blot - Anti-VPS28 antibody [EPR9880] (ab154793)
      All lanes : Anti-VPS28 antibody [EPR9880] (ab154793) at 1/1000 dilution (unpurified)

      Lane 1 : HepG2 cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : LnCaP cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 25 kDa

    • Immunoprecipitation - Anti-VPS28 antibody [EPR9880] (ab154793)
      Immunoprecipitation - Anti-VPS28 antibody [EPR9880] (ab154793)

      ab154793 (unpurified) at 1/50 immunoprecipitating VPS28 in jurkat cell lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Immunoprecipitation - Anti-VPS28 antibody [EPR9880] (ab154793)
      Immunoprecipitation - Anti-VPS28 antibody [EPR9880] (ab154793)

      ab154793 (purified) at 1/50 immunoprecipitating VPS28 in jurkat cell lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Anti-VPS28 antibody [EPR9880] (ab154793)
      Anti-VPS28 antibody [EPR9880] (ab154793)

    Protocols

    • Western blot protocols
    • Immunoprecipitation protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab154793? Please let us know so that we can cite the reference in this datasheet.

    ab154793 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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