Recombinant
RabMAb

Recombinant Anti-VPS28 antibody [EPR9880] (ab154793)

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Overview

  • Product name

    Anti-VPS28 antibody [EPR9880]
    See all VPS28 primary antibodies

  • Description

    Rabbit monoclonal [EPR9880] to VPS28

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human VPS28. The exact sequence is proprietary.
    Database link: Q9UK41

  • Positive control

    • WB: HepG2, Jurkat and LnCaP cell lysates and mouse and rat brain tissue lysates. IP: Jurkat cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab154793 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 25 kDa.
IP 1/50.
  • Application notes
    Is unsuitable for IHC-P.

    • Target

    Images

    • Western blot - Anti-VPS28 antibody [EPR9880] (ab154793)
      Western blot - Anti-VPS28 antibody [EPR9880] (ab154793)
      Anti-VPS28 antibody [EPR9880] (ab154793) at 1/2000 dilution (unpurified) + HepG2 cell lysate at 10 µg

      Secondary
      Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size: 25 kDa
      Observed band size: 25 kDa



      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Western blot - Anti-VPS28 antibody [EPR9880] (ab154793)
      Western blot - Anti-VPS28 antibody [EPR9880] (ab154793)
      Anti-VPS28 antibody [EPR9880] (ab154793) at 1/1500 dilution (purified) + HepG2 cell lysate at 10 µg

      Secondary
      Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size: 25 kDa
      Observed band size: 25 kDa



      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Western blot - Anti-VPS28 antibody [EPR9880] (ab154793)
      Western blot - Anti-VPS28 antibody [EPR9880] (ab154793)
      All lanes : Anti-VPS28 antibody [EPR9880] (ab154793) at 1/2000 dilution (unpurified)

      Lane 1 : Mouse brain tissue lysate
      Lane 2 : Rat brain tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size: 25 kDa
      Observed band size: 25 kDa



      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Western blot - Anti-VPS28 antibody [EPR9880] (ab154793)
      Western blot - Anti-VPS28 antibody [EPR9880] (ab154793)
      All lanes : Anti-VPS28 antibody [EPR9880] (ab154793) at 1/1500 dilution (purified)

      Lane 1 : Mouse brain tissue lysate
      Lane 2 : Rat brain tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size: 25 kDa
      Observed band size: 25 kDa



      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Western blot - Anti-VPS28 antibody [EPR9880] (ab154793)
      Western blot - Anti-VPS28 antibody [EPR9880] (ab154793)
      All lanes : Anti-VPS28 antibody [EPR9880] (ab154793) at 1/1000 dilution (unpurified)

      Lane 1 : HepG2 cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : LnCaP cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 25 kDa

    • Immunoprecipitation - Anti-VPS28 antibody [EPR9880] (ab154793)
      Immunoprecipitation - Anti-VPS28 antibody [EPR9880] (ab154793)

      ab154793 (unpurified) at 1/50 immunoprecipitating VPS28 in jurkat cell lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Immunoprecipitation - Anti-VPS28 antibody [EPR9880] (ab154793)
      Immunoprecipitation - Anti-VPS28 antibody [EPR9880] (ab154793)

      ab154793 (purified) at 1/50 immunoprecipitating VPS28 in jurkat cell lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    References

    ab154793 has not yet been referenced specifically in any publications.

    Publishing research using ab154793? Please let us know so that we can cite the reference in this datasheet.

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