Recombinant Anti-VPS28 antibody [EPR9880] (ab154793)
![Western blot - Anti-VPS28 antibody [EPR9880] (ab154793)](https://www.abcam.com/ps/products/154/ab154793/Images/ab154793-240899-anti-vps28-antibody-epr9880-western-blot.jpg "Western blot - Anti-VPS28 antibody [EPR9880] (ab154793)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9880] to VPS28
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Overview
-
Product name
Anti-VPS28 antibody [EPR9880]
See all VPS28 primary antibodies -
Description
Rabbit monoclonal [EPR9880] to VPS28 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB MouseHuman
-
Immunogen
Synthetic peptide within Human VPS28. The exact sequence is proprietary.
Database link: Q9UK41 -
Positive control
- WB: HepG2, Jurkat and LnCaP cell lysates and mouse and rat brain tissue lysates. IP: Jurkat cell lysate.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9880 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
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Isotype control
-
Positive Controls
- Mouse brain tissue lysate - total protein (ab30151)
- Rat brain cerebellum tissue lysate - total protein (ab4032)
- Mouse brain tissue lysate - total protein (0 days) (ab7188)
- Mouse brain tissue lysate - total protein (14 days) (ab7189)
- Mouse brain tissue lysate - total protein (7 days) (ab7190)
- Jurkat whole cell lysate (ab7899)
-
Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154793 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IP |
Human
|
| WB |
Mouse
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 25 kDa.
|
|
| IP |
1/50.
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 25 kDa. |
|
IP
1/50. |
Target
-
Function
Component of the ESCRT-I complex, a regulator of vesicular trafficking process. -
Sequence similarities
Belongs to the VPS28 family.
Contains 1 VPS28 C-terminal domain.
Contains 1 VPS28 N-terminal domain. -
Cellular localization
Cell membrane. Late endosome membrane. - Information by UniProt
-
Database links
- Entrez Gene: 51160 Human
- Entrez Gene: 66914 Mouse
- Entrez Gene: 300052 Rat
- Omim: 611952 Human
- SwissProt: Q9UK41 Human
- SwissProt: Q9D1C8 Mouse
- SwissProt: B5DEN9 Rat
- Unigene: 418175 Human
see all -
Alternative names
- ESCRT-I complex subunit VPS28 antibody
- H-Vps28 antibody
- Vacuolar protein sorting 28 antibody
see all
Images
-
Western blot - Anti-VPS28 antibody [EPR9880] (ab154793)Anti-VPS28 antibody [EPR9880] (ab154793) at 1/2000 dilution (unpurified) + HepG2 cell lysate at 10 µg
Secondary
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
-
![Western blot - Anti-VPS28 antibody [EPR9880] (ab154793)](https://www.abcam.com/ps/products/154/ab154793/Images/ab154793-240900-anti-vps28-antibody-epr9880-western-blot.jpg)
Western blot - Anti-VPS28 antibody [EPR9880] (ab154793)Anti-VPS28 antibody [EPR9880] (ab154793) at 1/1500 dilution (purified) + HepG2 cell lysate at 10 µg
Secondary
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
-
![Western blot - Anti-VPS28 antibody [EPR9880] (ab154793)](https://www.abcam.com/ps/products/154/ab154793/Images/ab154793-240901-anti-vps28-antibody-epr9880-western-blot.jpg)
Western blot - Anti-VPS28 antibody [EPR9880] (ab154793)All lanes : Anti-VPS28 antibody [EPR9880] (ab154793) at 1/2000 dilution (unpurified)
Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
-
![Western blot - Anti-VPS28 antibody [EPR9880] (ab154793)](https://www.abcam.com/ps/products/154/ab154793/Images/ab154793-240902-anti-vps28-antibody-epr9880-western-blot.jpg)
Western blot - Anti-VPS28 antibody [EPR9880] (ab154793)All lanes : Anti-VPS28 antibody [EPR9880] (ab154793) at 1/1500 dilution (purified)
Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
-
![Western blot - Anti-VPS28 antibody [EPR9880] (ab154793)](https://www.abcam.com/ps/products/154/ab154793/Images/ab154793-161286-anti-vps28-antibody-epr9880-western-blot.jpg)
Western blot - Anti-VPS28 antibody [EPR9880] (ab154793)All lanes : Anti-VPS28 antibody [EPR9880] (ab154793) at 1/1000 dilution (unpurified)
Lane 1 : HepG2 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : LnCaP cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 25 kDa -
![Immunoprecipitation - Anti-VPS28 antibody [EPR9880] (ab154793)](https://www.abcam.com/ps/products/154/ab154793/Images/ab154793-240903-anti-vps28-antibody-epr9880-immunoprecipitation.jpg)
Immunoprecipitation - Anti-VPS28 antibody [EPR9880] (ab154793)ab154793 (unpurified) at 1/50 immunoprecipitating VPS28 in jurkat cell lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
-
![Immunoprecipitation - Anti-VPS28 antibody [EPR9880] (ab154793)](https://www.abcam.com/ps/products/154/ab154793/Images/ab154793-240904-anti-vps28-antibody-epr9880-immunoprecipitation.jpg)
Immunoprecipitation - Anti-VPS28 antibody [EPR9880] (ab154793)ab154793 (purified) at 1/50 immunoprecipitating VPS28 in jurkat cell lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
-
![Anti-VPS28 antibody [EPR9880] (ab154793)](https://www.abcam.com/ps/products/154/ab154793/Images/ab154793-8-benefits-of-recombinant-antibodies.png)
Anti-VPS28 antibody [EPR9880] (ab154793)
Protocols
Datasheets and documents
References (0)
ab154793 has not yet been referenced specifically in any publications.
