Product nameAnti-VPS28 antibody [EPR9881(B)]
See all VPS28 primary antibodies
DescriptionRabbit monoclonal [EPR9881(B)] to VPS28
Tested applicationsSuitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
Unsuitable for: IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human VPS28.
- HepG2, Jurkat, Human fetal brain, A549 and Human fetal liver cell lysates; Human kidney tissue; HepG2 cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab167172 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 25 kDa.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/250.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionComponent of the ESCRT-I complex, a regulator of vesicular trafficking process.
Sequence similaritiesBelongs to the VPS28 family.
Contains 1 VPS28 C-terminal domain.
Contains 1 VPS28 N-terminal domain.
Cellular localizationCell membrane. Late endosome membrane.
- Information by UniProt
- ESCRT-I complex subunit VPS28 antibody
- H-Vps28 antibody
- Vacuolar protein sorting 28 antibody
All lanes : Anti-VPS28 antibody [EPR9881(B)] (ab167172) at 1/1000 dilution
Lane 1 : HepG2 cell lysates
Lane 2 : Jurkat cell lysates
Lane 3 : Human fetal brain lysates
Lane 4 : A549 cell lysates
Lane 5 : Human fetal liver lysates
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 25 kDa
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling VPS28 with ab167172 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunofluorescence analysis of HepG2 cells labeling VPS28 with ab167172 at 1/100 dilution.
ab167172 has not yet been referenced specifically in any publications.