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    vps29-antibody-ab236796.pdf

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Signal Transduction Protein Trafficking Vesicle Transport Regulation
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Anti-VPS29 antibody (ab236796)

  • Datasheet
  • SDS
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Western blot - Anti-VPS29 antibody (ab236796)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPS29 antibody (ab236796)
  • Immunocytochemistry/ Immunofluorescence - Anti-VPS29 antibody (ab236796)

Key features and details

  • Rabbit polyclonal to VPS29
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human VPS29 protein (ab124582)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-VPS29 antibody
    See all VPS29 primary antibodies
  • Description

    Rabbit polyclonal to VPS29
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human VPS29 aa 68-145.
    Database link: Q9UBQ0

  • Positive control

    • WB: MCF7, HepG2 and HeLa whole cell lysate (ab150035); Mouse heart and brain lysates; Rat liver and kidney lysates. IHC-P: Human adrenal gland tissue. ICC/IF: PC-3 cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Constituents: PBS, 50% Glycerol, 0.03% Proclin 300
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity >95%.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Regulation
    • Signal Transduction
    • Protein Trafficking
    • Golgi Proteins

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HepG2 whole cell lysate (ab166833)
    • MCF7 whole cell lysate (ab29537)
    • HeLa whole cell lysate (ab29545)
    • Mouse brain tissue lysate - total protein (ab30151)
    • Mouse heart normal tissue lysate - total protein (ab30291)
    • Mouse brain tissue lysate - total protein (ab4022)
  • Recombinant Protein

    • Recombinant Human VPS29 protein (ab124582)
  • Related Products

    • Recombinant Human VPS29 protein (ab124582)

Applications

Our Abpromise guarantee covers the use of ab236796 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/5000. Predicted molecular weight: 21 kDa.
IHC-P 1/20 - 1/200.
ICC/IF 1/50 - 1/200.

Target

  • Function

    Essential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA). Has low protein phosphatase activity towards a serine-phosphorylated peptide derived from IGF2R (in vitro).
  • Tissue specificity

    Ubiquitous. Highly expressed in heart, lung, placenta, spleen, peripheral blood leukocytes, thymus, colon skeletal muscle, kidney and brain.
  • Sequence similarities

    Belongs to the VPS29 family.
  • Cellular localization

    Cytoplasm. Membrane. Endosome membrane.
  • Target information above from: UniProt accession Q9UBQ0 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 51699 Human
    • Entrez Gene: 56433 Mouse
    • Entrez Gene: 288666 Rat
    • Omim: 606932 Human
    • SwissProt: Q9UBQ0 Human
    • SwissProt: Q9QZ88 Mouse
    • SwissProt: B2RZ78 Rat
    • Unigene: 600114 Human
    • Unigene: 216528 Mouse
    • Unigene: 445844 Mouse
    • Unigene: 2547 Rat
    see all
  • Alternative names

    • DC15 antibody
    • DC7 antibody
    • DKFZp564F0223 antibody
    • FLJ20492 antibody
    • hVPS29 antibody
    • PEP11 antibody
    • PEP11 homolog antibody
    • Retromer protein antibody
    • Vacuolar protein sorting 29 antibody
    • Vacuolar protein sorting 29 (yeast homolog) antibody
    • Vacuolar protein sorting 29 (yeast) antibody
    • Vacuolar protein sorting 29 homolog (S. cerevisiae) antibody
    • Vacuolar protein sorting 29 homolog antibody
    • Vacuolar protein sorting associated protein 29 antibody
    • Vacuolar protein sorting-associated protein 29 antibody
    • Vacuolar sorting protein VPS29/PEP11 antibody
    • Vesicle protein sorting 29 antibody
    • VPS 29 antibody
    • VPS29 antibody
    • VPS29 retromer complex component antibody
    • VPS29_HUMAN antibody
    • x 007 protein antibody
    see all

Images

  • Western blot - Anti-VPS29 antibody (ab236796)
    Western blot - Anti-VPS29 antibody (ab236796)
    All lanes : Anti-VPS29 antibody (ab236796) at 1/500 dilution

    Lane 1 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
    Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 4 : Mouse heart lysate
    Lane 5 : Mouse brain lysate
    Lane 6 : Rat liver lysate
    Lane 7 : Rat kidney lysate

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 21 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPS29 antibody (ab236796)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPS29 antibody (ab236796)

    Paraffin-embedded human adrenal gland tissue stained for VPS29 using ab236796 at 1/100 dilution in immunohistochemical analysis.

  • Immunocytochemistry/ Immunofluorescence - Anti-VPS29 antibody (ab236796)
    Immunocytochemistry/ Immunofluorescence - Anti-VPS29 antibody (ab236796)

    PC-3 (human prostate adenocarcinoma cell line) cells stained for VPS29 (green) using ab236796 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab236796? Please let us know so that we can cite the reference in this datasheet.

    ab236796 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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