Product nameAnti-VPS29 antibody
See all VPS29 primary antibodies
DescriptionRabbit polyclonal to VPS29
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment corresponding to Human VPS29 aa 68-145.
Database link: Q9UBQ0
- WB: MCF7, HepG2 and HeLa whole cell lysate (ab150035); Mouse heart and brain lysates; Rat liver and kidney lysates. IHC-P: Human adrenal gland tissue. ICC/IF: PC-3 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.03% Proclin
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
- HeLa whole cell lysate (ab150035)
- HepG2 whole cell lysate (ab166833)
- MCF7 whole cell lysate (ab29537)
- HeLa whole cell lysate (ab29545)
- Mouse brain tissue lysate - total protein (ab30151)
- Mouse heart normal tissue lysate - total protein (ab30291)
- MCF7 whole cell lysate (ab3871)
- Mouse brain tissue lysate - total protein (ab4022)
- HepG2 whole cell lysate (ab7900)
Our Abpromise guarantee covers the use of ab236796 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000. Predicted molecular weight: 21 kDa.|
|IHC-P||1/20 - 1/200.|
|ICC/IF||1/50 - 1/200.|
FunctionEssential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA). Has low protein phosphatase activity towards a serine-phosphorylated peptide derived from IGF2R (in vitro).
Tissue specificityUbiquitous. Highly expressed in heart, lung, placenta, spleen, peripheral blood leukocytes, thymus, colon skeletal muscle, kidney and brain.
Sequence similaritiesBelongs to the VPS29 family.
Cellular localizationCytoplasm. Membrane. Endosome membrane.
- Information by UniProt
- DC15 antibody
- DC7 antibody
- DKFZp564F0223 antibody
All lanes : Anti-VPS29 antibody (ab236796) at 1/500 dilution
Lane 1 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : Mouse heart lysate
Lane 5 : Mouse brain lysate
Lane 6 : Rat liver lysate
Lane 7 : Rat kidney lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 21 kDa
Paraffin-embedded human adrenal gland tissue stained for VPS29 using ab236796 at 1/100 dilution in immunohistochemical analysis.
PC-3 (human prostate adenocarcinoma cell line) cells stained for VPS29 (green) using ab236796 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
ab236796 has not yet been referenced specifically in any publications.