Anti-VPS29 antibody (ab236796)
Key features and details
- Rabbit polyclonal to VPS29
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-VPS29 antibody
See all VPS29 primary antibodies -
Description
Rabbit polyclonal to VPS29 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human VPS29 aa 68-145.
Database link: Q9UBQ0 -
Positive control
- WB: MCF7, HepG2 and HeLa whole cell lysate (ab150035); Mouse heart and brain lysates; Rat liver and kidney lysates. IHC-P: Human adrenal gland tissue. ICC/IF: PC-3 cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: PBS, 50% Glycerol, 0.03% Proclin 300 -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab236796 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/5000. Predicted molecular weight: 21 kDa. | |
| IHC-P | 1/20 - 1/200. | |
| ICC/IF | 1/50 - 1/200. |
Target
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Function
Essential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA). Has low protein phosphatase activity towards a serine-phosphorylated peptide derived from IGF2R (in vitro). -
Tissue specificity
Ubiquitous. Highly expressed in heart, lung, placenta, spleen, peripheral blood leukocytes, thymus, colon skeletal muscle, kidney and brain. -
Sequence similarities
Belongs to the VPS29 family. -
Cellular localization
Cytoplasm. Membrane. Endosome membrane. - Information by UniProt
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Database links
- Entrez Gene: 51699 Human
- Entrez Gene: 56433 Mouse
- Entrez Gene: 288666 Rat
- Omim: 606932 Human
- SwissProt: Q9UBQ0 Human
- SwissProt: Q9QZ88 Mouse
- SwissProt: B2RZ78 Rat
- Unigene: 600114 Human
see all -
Alternative names
- DC15 antibody
- DC7 antibody
- DKFZp564F0223 antibody
see all
Images
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Western blot - Anti-VPS29 antibody (ab236796)All lanes : Anti-VPS29 antibody (ab236796) at 1/500 dilution
Lane 1 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : Mouse heart lysate
Lane 5 : Mouse brain lysate
Lane 6 : Rat liver lysate
Lane 7 : Rat kidney lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 21 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPS29 antibody (ab236796)Paraffin-embedded human adrenal gland tissue stained for VPS29 using ab236796 at 1/100 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-VPS29 antibody (ab236796)PC-3 (human prostate adenocarcinoma cell line) cells stained for VPS29 (green) using ab236796 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab236796 has not yet been referenced specifically in any publications.
