Anti-VPS29 antibody (ab236796)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-VPS29 antibody
See all VPS29 primary antibodies -
Description
Rabbit polyclonal to VPS29 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human VPS29 aa 68-145.
Database link: Q9UBQ0 -
Positive control
- WB: MCF7, HepG2 and HeLa whole cell lysate (ab150035); Mouse heart and brain lysates; Rat liver and kidney lysates. IHC-P: Human adrenal gland tissue. ICC/IF: PC-3 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin
Constituents: PBS, 50% Glycerol -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
- HeLa whole cell lysate (ab150035)
- HepG2 whole cell lysate (ab166833)
- MCF7 whole cell lysate (ab29537)
- HeLa whole cell lysate (ab29545)
- Mouse brain tissue lysate - total protein (ab30151)
- Mouse heart normal tissue lysate - total protein (ab30291)
- MCF7 whole cell lysate (ab3871)
- Mouse brain tissue lysate - total protein (ab4022)
- HepG2 whole cell lysate (ab7900)
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab236796 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/5000. Predicted molecular weight: 21 kDa. | |
| IHC-P | 1/20 - 1/200. | |
| ICC/IF | 1/50 - 1/200. |
Target
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Function
Essential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA). Has low protein phosphatase activity towards a serine-phosphorylated peptide derived from IGF2R (in vitro). -
Tissue specificity
Ubiquitous. Highly expressed in heart, lung, placenta, spleen, peripheral blood leukocytes, thymus, colon skeletal muscle, kidney and brain. -
Sequence similarities
Belongs to the VPS29 family. -
Cellular localization
Cytoplasm. Membrane. Endosome membrane. - Information by UniProt
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Database links
- Entrez Gene: 51699 Human
- Entrez Gene: 56433 Mouse
- Entrez Gene: 288666 Rat
- Omim: 606932 Human
- SwissProt: Q9UBQ0 Human
- SwissProt: Q9QZ88 Mouse
- SwissProt: B2RZ78 Rat
- Unigene: 600114 Human
see all -
Alternative names
- DC15 antibody
- DC7 antibody
- DKFZp564F0223 antibody
see all
Images
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Western blot - Anti-VPS29 antibody (ab236796)All lanes : Anti-VPS29 antibody (ab236796) at 1/500 dilution
Lane 1 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : Mouse heart lysate
Lane 5 : Mouse brain lysate
Lane 6 : Rat liver lysate
Lane 7 : Rat kidney lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 21 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPS29 antibody (ab236796)Paraffin-embedded human adrenal gland tissue stained for VPS29 using ab236796 at 1/100 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-VPS29 antibody (ab236796)PC-3 (human prostate adenocarcinoma cell line) cells stained for VPS29 (green) using ab236796 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
References
ab236796 has not yet been referenced specifically in any publications.
