Anti-VPS34 antibody (ab233437)
Key features and details
- Rabbit polyclonal to VPS34
- Suitable for: WB, IHC-P
- Reacts with: Human, Pig
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-VPS34 antibody
See all VPS34 primary antibodies -
Description
Rabbit polyclonal to VPS34 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human, Pig
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment corresponding to Human VPS34 aa 600 to the C-terminus. Expressed in E.coli.
Database link: Q8NEB9 -
Positive control
- WB: Recombinant human VPS34 protein; HeLa cell lysate; Pig brain lysate. IHC-P: Human breast cancer, brain, liver and pancreas tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab233437 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 102 kDa.
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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Notes |
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WB
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 102 kDa. |
IHC-P
Use a concentration of 5 - 20 µg/ml. |
Target
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Function
Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate. Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for the abcission step in cytokinesis. -
Tissue specificity
Ubiquitously expressed, with a highest expression in skeletal muscle. -
Sequence similarities
Belongs to the PI3/PI4-kinase family.
Contains 1 PI3K/PI4K domain. -
Cellular localization
Midbody. - Information by UniProt
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Database links
- Entrez Gene: 5289 Human
- Entrez Gene: 225326 Mouse
- Entrez Gene: 503700 Pig
- Entrez Gene: 65052 Rat
- Omim: 602609 Human
- SwissProt: Q8NEB9 Human
- SwissProt: Q6PF93 Mouse
- SwissProt: Q5D891 Pig
see all -
Alternative names
- hVps34 antibody
- MGC61518 antibody
- Phosphatidylinositol 3 kinase catalytic subunit type 3 antibody
see all
Images
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Anti-VPS34 antibody (ab233437) at 3 µg/ml + Recombinant human VPS34 protein
Secondary
HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 102 kDa -
All lanes : Anti-VPS34 antibody (ab233437) at 3 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : Pig brain lysate
Secondary
All lanes : HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 102 kDa -
Formalin-fixed, paraffin-embedded human breast cancer tissue stained for VPS34 using ab233437 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded human cerebrum tissue stained for VPS34 using ab233437 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded human liver tissue stained for VPS34 using ab233437 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded human pancreatic cancer tissue stained for VPS34 using ab233437 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab233437 has not yet been referenced specifically in any publications.