Product nameAnti-VPS34 antibody [EPR5301]
See all VPS34 primary antibodies
DescriptionRabbit monoclonal [EPR5301] to VPS34
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human VPS34 aa 800-900. The exact sequence is proprietary.
- L6, C2C12, 293T, SHYSY-5Y cell lysates; Mouse brain tissue lysate; Human gastric adenocarcinoma tissue
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 3.27 x 10 -10 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab124905 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 102 kDa).|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionCatalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate. Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for the abcission step in cytokinesis.
Tissue specificityUbiquitously expressed, with a highest expression in skeletal muscle.
Sequence similaritiesBelongs to the PI3/PI4-kinase family.
Contains 1 PI3K/PI4K domain.
- Information by UniProt
- hVps34 antibody
- MGC61518 antibody
- Phosphatidylinositol 3 kinase catalytic subunit type 3 antibody
All lanes : Anti-VPS34 antibody [EPR5301] (ab124905) at 1/1000 dilution
Lane 1 : L6 cell lysate
Lane 2 : C2C12 cell lysate
Lane 3 : 293T cell lysate
Lane 4 : Mouse brain tissue lysate
Lane 5 : SHSY-5Y cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 102 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
ab124905, at 1/50 dilution, staining PI 3 Kinase in paraffin-embedded Human gastric adenocarcinoma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
This product has been referenced in:
- Tan S et al. ß-Arrestin1 enhances liver fibrosis through autophagy-mediated Snail signaling. FASEB J 33:2000-2016 (2019). Read more (PubMed: 30216111) »
- Zhang Q et al. Regulation of TGF-ß1 on PI3KC3 and its role in hypertension-induced vascular injuries. Exp Ther Med 17:1717-1727 (2019). Read more (PubMed: 30783440) »