Product nameAnti-VPS34 antibody [EPR5301]
See all VPS34 primary antibodies
DescriptionRabbit monoclonal [EPR5301] to VPS34
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human VPS34 aa 800-900. The exact sequence is proprietary.
- L6, C2C12, 293T, SHYSY-5Y cell lysates; Mouse brain tissue lysate; Human gastric adenocarcinoma tissue
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 3.27 x 10 -10 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab124905 in the following tested applications.
|WB||1/1000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 102 kDa).|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionCatalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate. Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for the abcission step in cytokinesis.
Tissue specificityUbiquitously expressed, with a highest expression in skeletal muscle.
Sequence similaritiesBelongs to the PI3/PI4-kinase family.
Contains 1 PI3K/PI4K domain.
- Information by UniProt
- hVps34 antibody
- MGC61518 antibody
- Phosphatidylinositol 3 kinase catalytic subunit type 3 antibody
All lanes : Anti-VPS34 antibody [EPR5301] (ab124905) at 1/1000 dilution
Lane 1 : L6 cell lysate
Lane 2 : C2C12 cell lysate
Lane 3 : 293T cell lysate
Lane 4 : Mouse brain tissue lysate
Lane 5 : SHSY-5Y cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 102 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
ab124905, at 1/50 dilution, staining PI 3 Kinase in paraffin-embedded Human gastric adenocarcinoma tissue by Immunohistochemistry.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
This product has been referenced in:
- Tan S et al. ß-Arrestin1 enhances liver fibrosis through autophagy-mediated Snail signaling. FASEB J 33:2000-2016 (2019). Read more (PubMed: 30216111) »
- Zhang Q et al. Regulation of TGF-ß1 on PI3KC3 and its role in hypertension-induced vascular injuries. Exp Ther Med 17:1717-1727 (2019). Read more (PubMed: 30783440) »