Overview

  • Product name

  • Description

    Rabbit polyclonal to VPS35
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 733-796 (KENDAVTIQV LNQLIQKIRE DLPNLESSEE TEQINKHFHN TLEHLRLRRE SPESEGPIYE GLIL) of Human VPS35 (NP_060676).

  • Positive control

    • H1299, HeLa S3, HepG2, MOLT4 and Raji cell lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab97545 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 92 kDa.

Target

  • Function

    Essential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA).
  • Tissue specificity

    Ubiquitous. Highly expressed in heart, brain, placenta, skeletal muscle, spleen, thymus, testis, ovary, small intestine, kidney and colon.
  • Sequence similarities

    Belongs to the VPS35 family.
  • Cellular localization

    Cytoplasm. Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • DKFZp434E1211 antibody
    • DKFZp434P1672 antibody
    • FLJ10752 antibody
    • FLJ13588 antibody
    • FLJ20388 antibody
    • hVPS35 antibody
    • Maternal embryonic 3 antibody
    • Maternal-embryonic 3 antibody
    • MEM 3 antibody
    • MEM3 antibody
    • PARK17 antibody
    • TCCCTA00141 antibody
    • Vacuolar protein sorting 35 (yeast) antibody
    • Vacuolar protein sorting 35 antibody
    • Vacuolar protein sorting 35 homolog antibody
    • Vacuolar protein sorting associated protein 35 antibody
    • Vacuolar protein sorting-associated protein 35 antibody
    • Vesicle protein sorting 35 antibody
    • VPS 35 antibody
    • VPS35 antibody
    • VPS35_HUMAN antibody
    see all

Images

  • All lanes : Anti-VPS35 antibody (ab97545) at 1/500 dilution

    Lane 1 : H1299 whole cell lysate
    Lane 2 : HeLaS3 whole cell lysate
    Lane 3 : HepG2 whole cell lysate
    Lane 4 : MOLT4 whole cell lysate
    Lane 5 : Raji whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 92 kDa



    7.5% SDS PAGE

References

This product has been referenced in:

  • Jimenez-Orgaz A  et al. Control of RAB7 activity and localization through the retromer-TBC1D5 complex enables RAB7-dependent mitophagy. EMBO J 37:235-254 (2018). Read more (PubMed: 29158324) »
  • Ansell-Schultz A  et al. Reduced retromer function results in the accumulation of amyloid-beta oligomers. Mol Cell Neurosci 93:18-26 (2018). Read more (PubMed: 30257187) »
See all 11 Publications for this product

Customer reviews and Q&As

Application
Immunocytochemistry
Sample
Mouse Cultured Cells (mouse cortical primary neuron culture DIV14)
Permeabilization
Yes - 0.2% Triton X-100
Specification
mouse cortical primary neuron culture DIV14
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Paraformaldehyde

Julie Nys

Verified customer

Submitted Nov 25 2015

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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