Overview

  • Product name
    Anti-VPS35 antibody [EPR11501(B)]
    See all VPS35 primary antibodies
  • Description
    Rabbit monoclonal [EPR11501(B)] to VPS35
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human VPS35. The exact sequence is proprietary.

  • Positive control
    • WB: HeLa, HepG2, Raji and MOLT4 cell lysates. IHC-P: Human kidney and human ovarian carcinoma tissue. ICC/IF: MOLT4 and wild-type HAP1 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab157220 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 92 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/250.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Essential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA).
    • Tissue specificity
      Ubiquitous. Highly expressed in heart, brain, placenta, skeletal muscle, spleen, thymus, testis, ovary, small intestine, kidney and colon.
    • Sequence similarities
      Belongs to the VPS35 family.
    • Cellular localization
      Cytoplasm. Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • DKFZp434E1211 antibody
      • DKFZp434P1672 antibody
      • FLJ10752 antibody
      • FLJ13588 antibody
      • FLJ20388 antibody
      • hVPS35 antibody
      • Maternal embryonic 3 antibody
      • Maternal-embryonic 3 antibody
      • MEM 3 antibody
      • MEM3 antibody
      • PARK17 antibody
      • TCCCTA00141 antibody
      • Vacuolar protein sorting 35 (yeast) antibody
      • Vacuolar protein sorting 35 antibody
      • Vacuolar protein sorting 35 homolog antibody
      • Vacuolar protein sorting associated protein 35 antibody
      • Vacuolar protein sorting-associated protein 35 antibody
      • Vesicle protein sorting 35 antibody
      • VPS 35 antibody
      • VPS35 antibody
      • VPS35_HUMAN antibody
      see all

    Images

    • Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: VPS35 knockout HAP1 cell lysate (20 µg)
      Lane 3: NIH/3T3 cell lysate (20 µg)
      Lane 4: A549 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab157220 observed at 91 kDa. Red - loading control, ab8245, observed at 37 kDa.


      ab157220 was shown to specifically react with VPS35 in wild-type HAP1 cells. No band was observed when VPS35 knockout samples were examined. Wild-type and VPS35 knockout samples were subjected to SDS-PAGE. ab157220 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

    • ab157220 staining VPS35 in wild-type HAP1 cells (top panel) and VPS35 knockout HAP1 cells (bottom panel). The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab157220 at 1/250 dilution and ab195889 at 1/250 dilution (shown in pseudo colour red) overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to Rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.

    • All lanes : Anti-VPS35 antibody [EPR11501(B)] (ab157220) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : Raji cell lysate
      Lane 4 : MOLT4 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 92 kDa

    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling VPS35 with ab157220 at 1/50 dilution.
    • Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling VPS35 with ab157220 at 1/50 dilution.
    • Flow cytometric analysis of permeabilized MOLT4 cells labeling VPS35 with ab157220 at 1/10 dilution (red) or a rabbit IgG (negative) (green).

    References

    This product has been referenced in:
    • Mir R  et al. The Parkinson's disease VPS35[D620N] mutation enhances LRRK2-mediated Rab protein phosphorylation in mouse and human. Biochem J 475:1861-1883 (2018). Read more (PubMed: 29743203) »
    • Ma Q  et al. SorCS2-mediated NR2A trafficking regulates motor deficits in Huntington's disease. JCI Insight 2:N/A (2017). WB ; Mouse . Read more (PubMed: 28469074) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab157220.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up