Recombinant HRP Anti-VPS35 antibody [EPR11501(B)] (ab215340)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR11501(B)] to VPS35
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Human
- Conjugation: HRP
Related conjugates and formulations
Overview
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Product name
HRP Anti-VPS35 antibody [EPR11501(B)]
See all VPS35 primary antibodies -
Description
HRP Rabbit monoclonal [EPR11501(B)] to VPS35 -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HepG2, A549, MOLT4, NIH3T3, Hap1 WT (shows pos.) and Hap1 VPS35 KO (shows neg.)
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: 1% BSA, 30% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR11501(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab215340 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/5000. Detects a band of approximately 92 kDa (predicted molecular weight: 92 kDa).
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Notes |
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WB
1/5000. Detects a band of approximately 92 kDa (predicted molecular weight: 92 kDa). |
Target
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Function
Essential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA). -
Tissue specificity
Ubiquitous. Highly expressed in heart, brain, placenta, skeletal muscle, spleen, thymus, testis, ovary, small intestine, kidney and colon. -
Sequence similarities
Belongs to the VPS35 family. -
Cellular localization
Cytoplasm. Membrane. - Information by UniProt
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Database links
- Entrez Gene: 100133770 Human
- Entrez Gene: 55737 Human
- Entrez Gene: 65114 Mouse
- Omim: 601501 Human
- SwissProt: Q96QK1 Human
- SwissProt: Q9EQH3 Mouse
- Unigene: 454528 Human
- Unigene: 296520 Mouse
see all -
Alternative names
- DKFZp434E1211 antibody
- DKFZp434P1672 antibody
- FLJ10752 antibody
see all
Images
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All lanes : HRP Anti-VPS35 antibody [EPR11501(B)] (ab215340) at 1/5000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : VPS35 knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 92 kDa
Exposure time: 1 minuteab215340 was shown to recognize VPS35 in wild-type HAP1 cells as signal was lost at the expected MW in VPS35 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and VPS35 knockout samples were subjected to SDS-PAGE. Ab215340 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680) loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.
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All lanes : HRP Anti-VPS35 antibody [EPR11501(B)] (ab215340) at 1/5000 dilution
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate at 10 µg
Lane 3 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate at 10 µg
Lane 4 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg
Lane 5 : HAP1 WT at 20 µg
Lane 6 : VPS35 knockout HAP1 at 20 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 92 kDa
Observed band size: 92 kDa
Exposure time: 20 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab215340 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab215340 has not yet been referenced specifically in any publications.