Recombinant Anti-VPS41 antibody [EPR13268] (ab181078)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13268] to VPS41
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-VPS41 antibody [EPR13268]
See all VPS41 primary antibodies -
Description
Rabbit monoclonal [EPR13268] to VPS41 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, human fetal brain, Mouse brain, RAW264.7 and Rat brain lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13268 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181078 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
1/1000 - 1/2000. Detects a band of approximately 99 kDa (predicted molecular weight: 99 kDa).
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Notes |
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WB
1/1000 - 1/2000. Detects a band of approximately 99 kDa (predicted molecular weight: 99 kDa). |
Target
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Relevance
VPS41 is required for vacuolar assembly and vacuolar traffic. Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. VSP41 is the human ortholog of yeast Vps41 protein which is also conserved in Drosophila, tomato, and Arabidopsis. Expression studies in yeast and human indicate that this protein may be involved in the formation and fusion of transport vesicles from the Golgi. Several transcript variants encoding different isoforms have been described for this gene, however, the full-length nature of not all is known. -
Cellular localization
Cytoplasmic face of coated pits and vesicles. -
Database links
- Entrez Gene: 27072 Human
- Entrez Gene: 218035 Mouse
- Entrez Gene: 306991 Rat
- Omim: 605485 Human
- SwissProt: P49754 Human
- SwissProt: Q5KU39 Mouse
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Alternative names
- HVPS41 antibody
- hVps41p antibody
- HVSP41 antibody
see all
Images
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All lanes : Anti-VPS41 antibody [EPR13268] (ab181078) at 1/1000 dilution (Purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : Human fetal brain lysates
Lane 3 : Mouse brain lysates
Lane 4 : RAW264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates
Lane 5 : Rat brain lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 99 kDa
Observed band size: 99 kDa -
All lanes : Anti-VPS41 antibody [EPR13268] (ab181078) at 1/1000 dilution
Lane 1 : Human fetal brain tissue lysate
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 99 kDa
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (4)
ab181078 has been referenced in 4 publications.
- Gu Y et al. Mammalian Atg8 proteins regulate lysosome and autolysosome biogenesis through SNAREs. EMBO J 38:e101994 (2019). PubMed: 31625181
- Marwaha R et al. The Rab7 effector PLEKHM1 binds Arl8b to promote cargo traffic to lysosomes. J Cell Biol 216:1051-1070 (2017). PubMed: 28325809
- Sindhwani A et al. Salmonella exploits the host endolysosomal tethering factor HOPS complex to promote its intravacuolar replication. PLoS Pathog 13:e1006700 (2017). PubMed: 29084291
- McLelland GL et al. Syntaxin-17 delivers PINK1/parkin-dependent mitochondrial vesicles to the endolysosomal system. J Cell Biol 214:275-91 (2016). PubMed: 27458136