Anti-VPS41 antibody [EPR13268] (ab181078)
- Datasheet
- Specific References (3)
- Protocols
Overview
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Product nameAnti-VPS41 antibody [EPR13268]
See all VPS41 primary antibodies -
DescriptionRabbit monoclonal [EPR13268] to VPS41
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Host speciesRabbit
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Tested applicationsSuitable for: WBmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide within Human VPS41 aa 800 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P49754 -
Positive control
- HeLa and Human fetal brain lysates.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR13268
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IsotypeIgG
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Research areas
Associated products
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab181078 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/2000. Detects a band of approximately 99 kDa (predicted molecular weight: 99 kDa). |
Target
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RelevanceVPS41 is required for vacuolar assembly and vacuolar traffic. Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. VSP41 is the human ortholog of yeast Vps41 protein which is also conserved in Drosophila, tomato, and Arabidopsis. Expression studies in yeast and human indicate that this protein may be involved in the formation and fusion of transport vesicles from the Golgi. Several transcript variants encoding different isoforms have been described for this gene, however, the full-length nature of not all is known.
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Cellular localizationCytoplasmic face of coated pits and vesicles.
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Database links
- Entrez Gene: 27072 Human
- Entrez Gene: 218035 Mouse
- Entrez Gene: 306991 Rat
- Omim: 605485 Human
- SwissProt: P49754 Human
- SwissProt: Q5KU39 Mouse
- Unigene: 592184 Human
- Unigene: 27389 Mouse
see all -
Alternative names
- HVPS41 antibody
- hVps41p antibody
- HVSP41 antibody
see all
Images
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![Western blot - Anti-VPS41 antibody [EPR13268] (ab181078)](https://www.abcam.com/ps/products/181/ab181078/Images/ab181078-213133-ab181078-wb.jpg)
Western blot - Anti-VPS41 antibody [EPR13268] (ab181078)All lanes : Anti-VPS41 antibody [EPR13268] (ab181078) at 1/1000 dilution
Lane 1 : Human fetal brain tissue lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 99 kDa
Protocols
References
This product has been referenced in:
- Marwaha R et al. The Rab7 effector PLEKHM1 binds Arl8b to promote cargo traffic to lysosomes. J Cell Biol 216:1051-1070 (2017). Read more (PubMed: 28325809) »
- Sindhwani A et al. Salmonella exploits the host endolysosomal tethering factor HOPS complex to promote its intravacuolar replication. PLoS Pathog 13:e1006700 (2017). Read more (PubMed: 29084291) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"