RecombinantRabMAb

Recombinant Anti-VPS41 antibody [EPR13268] (ab181078)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13268] to VPS41
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-VPS41 antibody [EPR13268]
    See all VPS41 primary antibodies

  • Description

    Rabbit monoclonal [EPR13268] to VPS41

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human VPS41 aa 800 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P49754

  • Positive control

    • WB: HeLa, human fetal brain, Mouse brain, RAW264.7 and Rat brain lysates

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR13268

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab181078 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 99 kDa (predicted molecular weight: 99 kDa).

Target

  • Relevance

    VPS41 is required for vacuolar assembly and vacuolar traffic. Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. VSP41 is the human ortholog of yeast Vps41 protein which is also conserved in Drosophila, tomato, and Arabidopsis. Expression studies in yeast and human indicate that this protein may be involved in the formation and fusion of transport vesicles from the Golgi. Several transcript variants encoding different isoforms have been described for this gene, however, the full-length nature of not all is known.

  • Cellular localization

    Cytoplasmic face of coated pits and vesicles.

  • Database links

    see all

  • Alternative names

    • HVPS41 antibody
    • hVps41p antibody
    • HVSP41 antibody
    • S53 antibody
    • Vacuolar assembly protein 41 antibody
    • Vacuolar protein sorting 41 (yeast homolog) antibody
    • Vacuolar protein sorting 41 (yeast) antibody
    • Vacuolar protein sorting 41 homolog (S. cerevisiae) antibody
    • Vacuolar protein sorting associated protein 41 homolog antibody
    • vacuolar protein sorting-associated protein 41 homolog antibody
    • VPS 41 antibody
    see all

Images

  • Western blot - Anti-VPS41 antibody [EPR13268] (ab181078)
    Western blot - Anti-VPS41 antibody [EPR13268] (ab181078)
    All lanes : Anti-VPS41 antibody [EPR13268] (ab181078) at 1/1000 dilution (Purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : Human fetal brain lysates
    Lane 3 : Mouse brain lysates
    Lane 4 : RAW264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates
    Lane 5 : Rat brain lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 99 kDa
    Observed band size: 99 kDa

  • Western blot - Anti-VPS41 antibody [EPR13268] (ab181078)
    Western blot - Anti-VPS41 antibody [EPR13268] (ab181078)
    All lanes : Anti-VPS41 antibody [EPR13268] (ab181078) at 1/1000 dilution

    Lane 1 : Human fetal brain tissue lysate
    Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 99 kDa

References (3)

Publishing research using ab181078? Please let us know so that we can cite the reference in this datasheet.

ab181078 has been referenced in 3 publications.

  • Marwaha R  et al. The Rab7 effector PLEKHM1 binds Arl8b to promote cargo traffic to lysosomes. J Cell Biol 216:1051-1070 (2017). PubMed: 28325809
  • Sindhwani A  et al. Salmonella exploits the host endolysosomal tethering factor HOPS complex to promote its intravacuolar replication. PLoS Pathog 13:e1006700 (2017). PubMed: 29084291
  • McLelland GL  et al. Syntaxin-17 delivers PINK1/parkin-dependent mitochondrial vesicles to the endolysosomal system. J Cell Biol 214:275-91 (2016). PubMed: 27458136

Customer reviews and Q&As

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Western blot abreview for Anti-VPS41 antibody [EPR13268]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Rat Cell lysate - other (PC12)
Gel Running Conditions
Reduced Denaturing (7.5% gel)
Loading amount
16 µg
Specification
PC12
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More

Cedric Asensio

Verified customer

Submitted Feb 13 2018

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