Overview

  • Product name

  • Description

    Goat polyclonal to VPS45A
  • Host species

    Goat
  • Tested applications

    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Pig, a wide range of other species
  • Immunogen

    Synthetic peptide: C-

    FQKKKPKEQQKLES

    , corresponding to internal sequence amino acids 294-307 of Human VPS45A.

  • Positive control

    • Human spleen tissue and Human placenta lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab129298 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/32000.
WB Use a concentration of 0.03 - 0.1 µg/ml. Predicted molecular weight: 65 kDa.
IHC-P Use a concentration of 2.5 - 3.75 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function

    May play a role in vesicle-mediated protein trafficking from the Golgi stack through the trans-Golgi network.
  • Tissue specificity

    Ubiquitous. Expression was highest in testis, heart and brain, intermediate in kidney, spleen, prostate, ovary, small intestine and thymus and low in lung, skeletal muscle, placenta, colon, pancreas, peripheral blood leukocytes and liver.
  • Sequence similarities

    Belongs to the STXBP/unc-18/SEC1 family.
  • Cellular localization

    Golgi apparatus membrane. Endosome membrane. Associated with Golgi/endosomal vesicles and the trans-Golgi network.
  • Information by UniProt
  • Database links

  • Alternative names

    • h-VPS45 antibody
    • H1 antibody
    • H1VPS45 antibody
    • HGNC:14579 antibody
    • hlVps45 antibody
    • Leucocyte vacuolar protein sorting 45 antibody
    • OTTHUMP00000014482 antibody
    • Vacuolar protein sorting 45 homolog (S. cerevisiae) antibody
    • Vacuolar protein sorting 45A (yeast homolog) antibody
    • Vacuolar protein sorting 45A antibody
    • Vacuolar protein sorting 45B (yeast) antibody
    • Vacuolar protein sorting-associated protein 45 antibody
    • Vps45 antibody
    • VPS45_HUMAN antibody
    • VPS45A antibody
    • VPS45B antibody
    • VPS54A antibody
    • VSP45 antibody
    • VSP45A antibody
    see all

Images

  • ab129208, at 3.75µg/ml, staining VPS45A in Formalin-fixed, Paraffin-embedded Human spleen tissue by Immunohistochemistry.
  • Anti-VPS45A antibody (ab129298) at 0.03 µg/ml + Human placenta lysate in RIPA buffer at 35 µg

    Developed using the ECL technique.

    Predicted band size: 65 kDa



    Primary ab129298 incubation was for one hour

References

ab129298 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab129298.
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