Key features and details
- Rabbit polyclonal to VPS4a
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-VPS4a antibody
DescriptionRabbit polyclonal to VPS4a
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
- WB: Mouse kidney and brain lysates; Rat gonadal lysate; U-87 MG and MCF7 whole cell lysates. IHC-P: Human lung and testis tissue.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229806 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/1000 - 1/5000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).|
FunctionInvolved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). Involved in cytokinesis.
Tissue specificityUbiquitously expressed.
Sequence similaritiesBelongs to the AAA ATPase family.
Contains 1 MIT domain.
DomainThe MIT domain serves as an adapter for ESCRT-III proteins. It forms an asymmetric three-helix bundle that binds amphipathic MIM (MIT interacting motif) helices along the groove between MIT helices 2 and 3 present in a subset of ESCRT-III proteins thus establishing the canonical MIM-MIT interaction. In an extended conformation along the groove between helices 1 and 3, also binds to a type-2 MIT interacting motif (MIM2).
Cellular localizationPrevacuolar compartment membrane. Late endosome membrane. Membrane-associated in the prevacuolar endosomal compartment. Localizes to the midbody of dividing cells. Localized in two distinct rings on either side of the Fleming body.
- Information by UniProt
- hVPS4 antibody
- Protein SKD2 antibody
- SKD1 antibody
All lanes : Anti-VPS4a antibody (ab229806) at 1/1000 dilution
Lane 1 : Mouse kidney lysate
Lane 2 : Rat gonadal lysate
Lane 3 : Mouse brain lysate
Lane 4 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
Lane 5 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 49 kDa
Paraffin-embedded human lung tissue stained for VPS4a with ab229806 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human testis tissue stained for VPS4a with ab229806 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229806 has not yet been referenced specifically in any publications.