Recombinant
RabMAb

Recombinant Anti-VPS4a antibody [EPR14545(B)] (ab180581)

Overview

  • Product name

    Anti-VPS4a antibody [EPR14545(B)]
    See all VPS4a primary antibodies
  • Description

    Rabbit monoclonal [EPR14545(B)] to VPS4a
  • Host species

    Rabbit
  • Specificity

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    within Human VPS4a aa 400 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9UN37

  • Positive control

    • WB: MCF-7, Hela, U87-MG cell lysates, mouse brain tissue lysate, mouse and rat testis lysates, Fetal brain lysate IHC-P: human testis tissue
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab180581 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 49 kDa.
IHC-P 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Function

      Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). Involved in cytokinesis.
    • Tissue specificity

      Ubiquitously expressed.
    • Sequence similarities

      Belongs to the AAA ATPase family.
      Contains 1 MIT domain.
    • Domain

      The MIT domain serves as an adapter for ESCRT-III proteins. It forms an asymmetric three-helix bundle that binds amphipathic MIM (MIT interacting motif) helices along the groove between MIT helices 2 and 3 present in a subset of ESCRT-III proteins thus establishing the canonical MIM-MIT interaction. In an extended conformation along the groove between helices 1 and 3, also binds to a type-2 MIT interacting motif (MIM2).
    • Cellular localization

      Prevacuolar compartment membrane. Late endosome membrane. Membrane-associated in the prevacuolar endosomal compartment. Localizes to the midbody of dividing cells. Localized in two distinct rings on either side of the Fleming body.
    • Information by UniProt
    • Database links

    • Alternative names

      • hVPS4 antibody
      • Protein SKD2 antibody
      • SKD1 antibody
      • SKD1 homolog antibody
      • SKD1A antibody
      • SKD2 antibody
      • Vacuolar protein sorting 4 homolog A antibody
      • Vacuolar protein sorting 4, yeast, homolog of, A antibody
      • Vacuolar protein sorting factor 4A antibody
      • Vacuolar protein sorting-associated protein 4A antibody
      • vacuolar sorting protein 4 antibody
      • VPS4 antibody
      • VPS4-1 antibody
      • Vps4a antibody
      • VPS4A_HUMAN antibody
      see all

    Images

    • All lanes : Anti-VPS4a antibody [EPR14545(B)] (ab180581) at 1/20000 dilution (Purified)

      Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
      Lane 2 : Mouse brain lysates
      Lane 3 : Mouse testis lysates
      Lane 4 : Rat testis lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 49 kDa
      Observed band size: 50 kDa
      why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human testis tissue sections labeling VPS4a with purified ab180581 at 1/2000 dilution (0.098 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    • All lanes : Anti-VPS4a antibody [EPR14545(B)] (ab180581) at 1/50000 dilution (unpurified)

      Lane 1 : Fetal brain lysate
      Lane 2 : MCF-7 cell lysate
      Lane 3 : Hela cell lysate
      Lane 4 : U87-MG cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated

      Predicted band size: 49 kDa

    • Immunohistochemical stainin gof paraffin-embedded Human testis tissue labeling VPS4a with ab180581 (unpurified) at 1/2000 dilution followed by prediluted ImmunoHistoprobe(Ready to use) HRP Polymer for Rabbit IgG.  Sample was counterstained with Hematoxylin.

    References

    ab180581 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    This product is known to not work in this application or species.
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 45 minute(s) · Concentration: 2% · Temperature: RT°C
    Sample
    African Green Monkey Cell (Vero cell)
    Specification
    Vero cell
    Permeabilization
    Yes - TritonX100 al 0,1%, Tween20
    Fixative
    Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Dec 19 2014

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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