Recombinant
RabMAb

Recombinant Anti-VPS4a antibody [EPR14545(B)] - BSA and Azide free (ab240226)

Overview

  • Product name

    Anti-VPS4a antibody [EPR14545(B)] - BSA and Azide free
    See all VPS4a primary antibodies
  • Description

    Rabbit monoclonal [EPR14545(B)] to VPS4a - BSA and Azide free
  • Host species

    Rabbit
  • Specificity

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human VPS4a aa 400 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9UN37

  • Positive control

    • IHC-P: human testis tissue
  • General notes

    Ab240226 is the carrier-free version of ab180581. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab240226 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab240226 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 49 kDa.
IHC-P Use at an assay dependent concentration.

See IHC antigen retrieval protocols.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Function

      Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). Involved in cytokinesis.
    • Tissue specificity

      Ubiquitously expressed.
    • Sequence similarities

      Belongs to the AAA ATPase family.
      Contains 1 MIT domain.
    • Domain

      The MIT domain serves as an adapter for ESCRT-III proteins. It forms an asymmetric three-helix bundle that binds amphipathic MIM (MIT interacting motif) helices along the groove between MIT helices 2 and 3 present in a subset of ESCRT-III proteins thus establishing the canonical MIM-MIT interaction. In an extended conformation along the groove between helices 1 and 3, also binds to a type-2 MIT interacting motif (MIM2).
    • Cellular localization

      Prevacuolar compartment membrane. Late endosome membrane. Membrane-associated in the prevacuolar endosomal compartment. Localizes to the midbody of dividing cells. Localized in two distinct rings on either side of the Fleming body.
    • Information by UniProt
    • Database links

    • Alternative names

      • hVPS4 antibody
      • Protein SKD2 antibody
      • SKD1 antibody
      • SKD1 homolog antibody
      • SKD1A antibody
      • SKD2 antibody
      • Vacuolar protein sorting 4 homolog A antibody
      • Vacuolar protein sorting 4, yeast, homolog of, A antibody
      • Vacuolar protein sorting factor 4A antibody
      • Vacuolar protein sorting-associated protein 4A antibody
      • vacuolar sorting protein 4 antibody
      • VPS4 antibody
      • VPS4-1 antibody
      • Vps4a antibody
      • VPS4A_HUMAN antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human testis tissue sections labeling VPS4a with purified ab180581 at 1/2000 dilution (0.098 µg/ml). Heat mediated antigen retrieval was performed using heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180581).

    • Immunohistochemical stainin gof paraffin-embedded Human testis tissue labeling VPS4a with ab180581 (unpurified) at 1/2000 dilution followed by prediluted ImmunoHistoprobe(Ready to use) HRP Polymer for Rabbit IgG.  Sample was counterstained with Hematoxylin.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180581).

    References

    ab240226 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab240226.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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