Recombinant Anti-VPS4B/MIG1 antibody [EPR9225] (ab137027)
![Western blot - Anti-VPS4B/MIG1 antibody [EPR9225] (ab137027)](https://www.abcam.com/ps/products/137/ab137027/Images/ab137027-151495-anti-vps4b-antibody-epr9225-western-blot.jpg "Western blot - Anti-VPS4B/MIG1 antibody [EPR9225] (ab137027)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9225] to VPS4B/MIG1
- Suitable for: WB, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-VPS4B/MIG1 antibody [EPR9225]
See all VPS4B/MIG1 primary antibodies -
Description
Rabbit monoclonal [EPR9225] to VPS4B/MIG1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cytmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human VPS4B/MIG1 aa 350-450. The exact sequence is proprietary.
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Positive control
- HeLa, 293T, Jurkat, and HepG2 whole cell lysate (ab7900); 293T cells
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as VPS4B
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9225 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab137027 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 49 kDa. | |
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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Function
Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). -
Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Belongs to the AAA ATPase family.
Contains 1 MIT domain. -
Domain
The MIT domain serves as an adapter for ESCRT-III proteins. It forms an asymmetric three-helix bundle that binds amphipathic MIM (MIT interacting motif) helices along the groove between MIT helices 2 and 3 present in a subset of ESCRT-III proteins thus establishing the canonical MIM-MIT interaction. In an extended conformation along the groove between helices 1 and 3, also binds to a type-2 MIT interacting motif (MIM2). -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Prevacuolar compartment membrane. Late endosome membrane. Membrane-associated in the prevacuolar endosomal compartment. Localized in HIV-1 particles purified from acutely infected cells. - Information by UniProt
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Database links
- Entrez Gene: 9525 Human
- Omim: 609983 Human
- SwissProt: O75351 Human
- Unigene: 126550 Human
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Alternative names
- 8030489C12Rik antibody
- Cell migration inducing 1 antibody
- Cell migration-inducing gene 1 protein antibody
see all
Images
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Western blot - Anti-VPS4B/MIG1 antibody [EPR9225] (ab137027)All lanes : Anti-VPS4B/MIG1 antibody [EPR9225] (ab137027) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 49 kDa -
![Flow Cytometry - Anti-VPS4B/MIG1 antibody [EPR9225] (ab137027)](https://www.abcam.com/ps/products/137/ab137027/Images/ab137027-151494-anti-vps4b-antibody-epr9225-flow-cytometry.jpg)
Flow Cytometry - Anti-VPS4B/MIG1 antibody [EPR9225] (ab137027)Flow Cytometric analysis of permeabilized 293T cells labelling VPS4B/MIG1 with ab137027 at 1/10 dilution (red). Rabbit IgG negative control is green.
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![Anti-VPS4B/MIG1 antibody [EPR9225] (ab137027)](https://www.abcam.com/ps/products/137/ab137027/Images/ab137027-3-benefits-of-recombinant-antibodies.png)
Anti-VPS4B/MIG1 antibody [EPR9225] (ab137027)
Datasheets and documents
References (1)
ab137027 has been referenced in 1 publication.
- Mierzwa BE et al. Dynamic subunit turnover in ESCRT-III assemblies is regulated by Vps4 to mediate membrane remodelling during cytokinesis. Nat Cell Biol 19:787-798 (2017). WB . PubMed: 28604678
