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Signal Transduction Protein Trafficking Organelle Proteins
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RecombinantRabMAb

Recombinant Anti-VPS4B/MIG1 antibody [EPR9225] (ab137027)

  • Datasheet
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Western blot - Anti-VPS4B/MIG1 antibody [EPR9225] (ab137027)
  • Flow Cytometry - Anti-VPS4B/MIG1 antibody [EPR9225] (ab137027)
  • Anti-VPS4B/MIG1 antibody [EPR9225] (ab137027)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9225] to VPS4B/MIG1
  • Suitable for: WB, Flow Cyt
  • Reacts with: Human

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Overview

  • Product name

    Anti-VPS4B/MIG1 antibody [EPR9225]
    See all VPS4B/MIG1 primary antibodies
  • Description

    Rabbit monoclonal [EPR9225] to VPS4B/MIG1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human VPS4B/MIG1 aa 350-450. The exact sequence is proprietary.

  • Positive control

    • HeLa, 293T, Jurkat, and HepG2 whole cell lysate (ab7900); 293T cells
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

     This product was previously labelled as VPS4B

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR9225
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Trafficking
    • Organelle Proteins

Associated products

  • Alternative Versions

    • Anti-VPS4B/MIG1 antibody [EPR9225] - BSA and Azide free (ab248775)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
    • Jurkat whole cell lysate (ab7899)
  • Recombinant Protein

    • Recombinant Human VPS4B/MIG1 protein (ab171483)

Applications

Our Abpromise guarantee covers the use of ab137027 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 49 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function

      Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses).
    • Tissue specificity

      Ubiquitously expressed.
    • Sequence similarities

      Belongs to the AAA ATPase family.
      Contains 1 MIT domain.
    • Domain

      The MIT domain serves as an adapter for ESCRT-III proteins. It forms an asymmetric three-helix bundle that binds amphipathic MIM (MIT interacting motif) helices along the groove between MIT helices 2 and 3 present in a subset of ESCRT-III proteins thus establishing the canonical MIM-MIT interaction. In an extended conformation along the groove between helices 1 and 3, also binds to a type-2 MIT interacting motif (MIM2).
    • Post-translational
      modifications

      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization

      Prevacuolar compartment membrane. Late endosome membrane. Membrane-associated in the prevacuolar endosomal compartment. Localized in HIV-1 particles purified from acutely infected cells.
    • Target information above from: UniProt accession O75351 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 9525 Human
      • Omim: 609983 Human
      • SwissProt: O75351 Human
      • Unigene: 126550 Human
      • Alternative names

        • 8030489C12Rik antibody
        • Cell migration inducing 1 antibody
        • Cell migration-inducing gene 1 protein antibody
        • MGC116271 antibody
        • MIG1 antibody
        • Protein SKD1 antibody
        • Skd1 antibody
        • SKD1B antibody
        • Suppressor of K(+) transport growth defect 1 antibody
        • Suppressor of K+ transport defect 1 antibody
        • Vacuolar protein sorting 4 homolog B (S. cerevisiae) antibody
        • Vacuolar protein sorting 4 homolog B antibody
        • Vacuolar protein sorting 4b antibody
        • Vacuolar protein sorting-associated protein 4B antibody
        • Vacuolar protein sorting-associating protein 4B antibody
        • VPS4 2 antibody
        • VPS42 antibody
        • Vps4b antibody
        • VPS4B_HUMAN antibody
        see all

      Images

      • Western blot - Anti-VPS4B/MIG1 antibody [EPR9225] (ab137027)
        Western blot - Anti-VPS4B/MIG1 antibody [EPR9225] (ab137027)
        All lanes : Anti-VPS4B/MIG1 antibody [EPR9225] (ab137027) at 1/1000 dilution

        Lane 1 : HeLa cell lysate
        Lane 2 : 293T cell lysate
        Lane 3 : Jurkat cell lysate
        Lane 4 : HepG2 cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 49 kDa

      • Flow Cytometry - Anti-VPS4B/MIG1 antibody [EPR9225] (ab137027)
        Flow Cytometry - Anti-VPS4B/MIG1 antibody [EPR9225] (ab137027)

        Flow Cytometric analysis of permeabilized 293T cells labelling VPS4B/MIG1 with ab137027 at 1/10 dilution (red). Rabbit IgG negative control is green.

      • Anti-VPS4B/MIG1 antibody [EPR9225] (ab137027)
        Anti-VPS4B/MIG1 antibody [EPR9225] (ab137027)

      Protocols

      • Western blot protocols
      • Flow cytometry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (1)

      Publishing research using ab137027? Please let us know so that we can cite the reference in this datasheet.

      ab137027 has been referenced in 1 publication.

      • Mierzwa BE  et al. Dynamic subunit turnover in ESCRT-III assemblies is regulated by Vps4 to mediate membrane remodelling during cytokinesis. Nat Cell Biol 19:787-798 (2017). WB . PubMed: 28604678

      Customer reviews and Q&As

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      There are currently no Customer reviews or Questions for ab137027.
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