Overview

  • Product name

    Anti-VPS52 antibody
  • Description

    Rabbit polyclonal to VPS52
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human VPS52 aa 277-377.
    Database link: Q8N1B4

  • Positive control

    • IHC-P: Human small intestine and appendix tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab238723 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance

    Vesicle mediated protein sorting is important for the distribution of intracellular molecules into distinct organelles. More than 40 vacuolar protein sorting (VPS) genes are involved in this process. VPS52 is similar to the yeast suppressor of actin mutations 2 gene. The yeast protein forms a subunit of the tetrameric Golgi-associated retrograde protein complex that is involved in vesicle trafficking from from both early and late endosomes, back to the trans-Golgi network. VPS52 is located on chromosome 6 in a head-to-head orientation with the gene encoding ribosomal protein S18.
  • Cellular localization

    Golgi apparatus; trans-Golgi network membrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein.
  • Database links

  • Alternative names

    • RP5 1033B10 antibody
    • ARE1 antibody
    • dJ1033B10.5 antibody
    • DKFZp547I194 antibody
    • RP5-1033B10 antibody
    • SAC2 antibody
    • SAC2 suppressor of actin mutations 2 like (yeast) antibody
    • SAC2 suppressor of actin mutations 2 like protein antibody
    • SACM2L antibody
    • Vacuolar protein sorting 52 (yeast) antibody
    • vacuolar protein sorting 52 homolog (S. cerevisiae) antibody
    • Vacuolar protein sorting associated protein 52 homolog antibody
    see all

Images

  • Paraffin-embedded human small intestine tissue stained for VPS52 using ab238723 at 1/200 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Paraffin-embedded human appendix tissue stained for VPS52 using ab238723 at 1/200 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

References

ab238723 has not yet been referenced specifically in any publications.

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