Key features and details
- Rabbit polyclonal to VPS53
- Suitable for: ICC/IF, WB, ICC
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-VPS53 antibody
See all VPS53 primary antibodies
DescriptionRabbit polyclonal to VPS53
Tested applicationsSuitable for: ICC/IF, WB, ICCmore details
Species reactivityReacts with: Mouse, Rat, Human
An 18 amino acid synthetic peptide from near the C terminus of Human VPS53 (EAW90658).
- 293 cells and cell lysate
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at 4°C (stable for up to 12 months).
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab106469 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 20 µg/ml.|
|WB||Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 80 kDa.|
|ICC||Use a concentration of 5 µg/ml.|
FunctionMay be involved in retrograde transport of early and late endosomes to the late Golgi.
Sequence similaritiesBelongs to the VPS53 family.
Cellular localizationGolgi apparatus > trans-Golgi network membrane. Endosome membrane.
- Information by UniProt
- FLJ10979 antibody
- FLJ41112 antibody
- FLJ61757 antibody
Immunofluorescence of VPS53 in 293 cells with ab106469 at 2.5 ug/mL.
Lane 1 : Anti-VPS53 antibody (ab106469) at 0.5 µg/ml
Lane 2 : Anti-VPS53 antibody (ab106469) at 1 µg/ml
All lanes : 293 cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 80 kDa
ab106469, at 5 µg/ml, staining VPS53 in 293 cells by Immunocytochemistry.
ab106469 has been referenced in 2 publications.
- Zhang Z et al. GZFLW Induces Apoptosis of Ectopic Endometrial Stromal Cells via Promoting VPS53 Protein Stability. Evid Based Complement Alternat Med 2018:1293630 (2018). PubMed: 30643524
- Masschaele D et al. RNF41 interacts with the VPS52 subunit of the GARP and EARP complexes. PLoS One 12:e0178132 (2017). ICC/IF . PubMed: 28542518