Overview

  • Product name

  • Description

    Rabbit polyclonal to VR1
  • Host species

    Rabbit
  • Specificity

    Immunohistochemical staining of VR1 in human glabrous skin using this antibody results in the staining of epidermal and dermal cells, as well as free nerve fibers and Merkel cells. Immunofluorescent staining of VR1 in human prostatic urethra results in fibrous staining within the epithelial cells.
  • Tested applications

    Suitable for: IHC-Fr, IHC-P, WB, ICC/IF, IHC-FrFlmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human VR1 aa 7-21.
    Sequence:

    TDLGAAADPLQKDTC


    (Peptide available as ab5894)

  • Positive control

    • IHC: human glabrous skin

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Primary antibody notes

    Vanilloid Receptor 1 (VR1), also known as capsaicin receptor, responds to both capsaicin, the pungent ingredient in hot peppers, and thermal stimuli within the noxious temperature range. This receptor is structurally related to the TRP family of ion channels and is predicted to contain 6 trans-membrane domains, a P-loop membrane domain and 3 ankyrin repeat domains. VR1 is highly permeable to divalent cations, including calcium and magnesium, as well as the monovalent cations sodium and potassium. Expression of VR1 appears to be limited to neurons within the sensory ganglia.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3487 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use a concentration of 1 µg/ml.
IHC-P Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 94 kDa.
ICC/IF Use a concentration of 1 µg/ml.
IHC-FrFl Use at an assay dependent concentration.

Target

  • Function

    Receptor-activated non-selective calcium permeant cation channel involved in detection of noxious chemical and thermal stimuli. Seems to mediate proton influx and may be involved in intracellular acidosis in nociceptive neurons. May be involved in mediation of inflammatory pain and hyperalgesia. Sensitized by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases, which involves PKC isozymes and PCL. Acts as ionotropic endocannabinoid receptor with central neuromodulatory effects. Triggers a form of long-term depression (TRPV1-LTD) mediated by the endocannabinoid anandamine in the hippocampus and nucleus accubens by affecting AMPA receptors endocytosis.
  • Tissue specificity

    Widely expressed at low levels. Expression is elevated in dorsal root ganglia. In skin, expressed in cutaneous sensory nerve fibers, mast cells, epidermal keratinocytes, dermal blood vessels, the inner root sheet and the infundibulum of hair follicles, differentiated sebocytes, sweat gland ducts, and the secretory portion of eccrine sweat glands (at protein level).
  • Sequence similarities

    Belongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV1 sub-subfamily.
    Contains 6 ANK repeats.
  • Domain

    The association domain (AD) is necessary for self-association.
  • Post-translational
    modifications

    Phosphorylation by PKA reverses capsaicin-induced dephosphorylation at multiple sites, probably including Ser-117 as a major phosphorylation site. Phoshphorylation by CAMKII seems to regulate binding to vanilloids. Phosphorylated and modulated by PKCM and probably PKCZ. Dephosphorylation by calcineurin seems to lead to receptor desensitization and phosphorylation by CAMKII recovers activity.
  • Cellular localization

    Cell junction > synapse > postsynaptic cell membrane. Cell projection > dendritic spine membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Capsaicin receptor antibody
    • DKFZp434K0220 antibody
    • osm 9 like TRP channel 1 antibody
    • Osm-9-like TRP channel 1 antibody
    • OTRPC1 antibody
    • Transient receptor potential cation channel subfamily V member 1 antibody
    • TRPV 1 antibody
    • Trpv1 antibody
    • TRPV1_HUMAN antibody
    • Vanilloid receptor 1 antibody
    • Vanilloid receptor subtype 1 antibody
    • VR 1 antibody
    • VR1 antibody
    see all

Images

  • Immunolocalization of VR1 in human glabrous skin using ab3487.

  • Immunohistochemistry (frozen sections) analysis of human skin tissue sections labeling VR1 (red) with ab3487 at 1/250. A biotin-conjugated donkey anti-rabbit IgG was used as the secondary antibody.

References

This product has been referenced in:

See all 6 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer

Thank your email. I am very sorry that the suggestions did not helpto improve the results. I must therefore conclude, that the vial you received must have been damaged - maybe during storage or transport. I would be pleased to replace this vial for you, by either a credit/refund or a replacement vial. Please let me know which optionyou prefer andwhether you would like to accept either a new vial of ab3487 or another alternative antibody from our catalog?
I apologize for the inconvenience. Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.

Read More

Answer

Thank you for contacting us. I am sorry to hear that this antibody is not providing satisfactory results.

We do not have a specific protocol for this antibody. We expect this antibody to work with a general IHC protocol after the classic optimisation steps. Looking at the information you provided, I would like to offerthe followingsuggestions to help optimize the results from ab3487. I would also appreciate if you can confirm some further details:

1.) Can you please confirm of which species the skin sections usedare coming from. We have tested this antibody so far only on human tissue. It might reacts with other species, however we need to test this first.

2.) I can recommend to incubate the antibody overnight at 4°C. This can increase the staining intensity.

3.) I can also recommend to test different blocking solutions. This can significantly improve the staining. I can recommend to test BSA and serum instead of milk. As serum you would use serum of the species of the secondary antibody (goat?). The researcher having submitted the feedback on this antibody, has used for example 10% serum. See his Abreview: https://www.abcam.com/index.html?datasheet=3487&tab=abreviews&intabreviewid=26192

4.) Can you confirm that the other reactives (e.g. the secondary antibody) do work fine when used with other primary rabbit antibodies?

5.) Have you tested other fixation methods as well, such as Methanol only or Paraformaldehyde? The user mentionned above, had used succesfully Methanol only, whereas the lab has tested the antibody also in paraformaldhyde fixation obtaining good results.

I hope these suggestions will help to identify the problem. Should the suggestions not improve the results, please do let me know.

In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund.

I hope this information is helpful, and I thank you for your cooperation.

Read More
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (skin, 3D skin model)
Specification
skin, 3D skin model
Fixative
Methanol
Permeabilization
Yes - 0.4% Triton X100
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Sep 21 2011

Application
Immunohistochemistry free floating
Sample
Human Tissue sections (skin)
Specification
skin

Abcam user community

Verified customer

Submitted Jun 10 2011

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