Overview

  • Product name
  • Description
    Rabbit polyclonal to VR1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Rat VR1 aa 819-838 (C terminal) conjugated to keyhole limpet haemocyanin (Glutaraldehyde).
    Sequence:

    CGSLKPEDAEVFKDSMVPGEK

  • Positive control
    • IHC-P: Rat dorsal ganglia.
  • General notes
    This antibody will stain a selected population of sensory neurons in the dorsal root ganglion. It is unsuitable for staining nerve fibres or terminals.

Properties

Applications

Our Abpromise guarantee covers the use of ab6166 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Predicted molecular weight: 94.976 kDa.
ICC/IF 1/50 - 1/100.
IHC-Fr 1/50 - 1/100.

See Peier et al.

IHC-P 1/1000 - 1/2000.

Use at 1/1000 – 1/2000 dilution for DAB staining and 1/100 – 1/200 dilution for immunofluorescence staining.

Target

  • Function
    Receptor-activated non-selective calcium permeant cation channel involved in detection of noxious chemical and thermal stimuli. Seems to mediate proton influx and may be involved in intracellular acidosis in nociceptive neurons. May be involved in mediation of inflammatory pain and hyperalgesia. Sensitized by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases, which involves PKC isozymes and PCL. Acts as ionotropic endocannabinoid receptor with central neuromodulatory effects. Triggers a form of long-term depression (TRPV1-LTD) mediated by the endocannabinoid anandamine in the hippocampus and nucleus accubens by affecting AMPA receptors endocytosis.
  • Tissue specificity
    Widely expressed at low levels. Expression is elevated in dorsal root ganglia. In skin, expressed in cutaneous sensory nerve fibers, mast cells, epidermal keratinocytes, dermal blood vessels, the inner root sheet and the infundibulum of hair follicles, differentiated sebocytes, sweat gland ducts, and the secretory portion of eccrine sweat glands (at protein level).
  • Sequence similarities
    Belongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV1 sub-subfamily.
    Contains 6 ANK repeats.
  • Domain
    The association domain (AD) is necessary for self-association.
  • Post-translational
    modifications
    Phosphorylation by PKA reverses capsaicin-induced dephosphorylation at multiple sites, probably including Ser-117 as a major phosphorylation site. Phoshphorylation by CAMKII seems to regulate binding to vanilloids. Phosphorylated and modulated by PKCM and probably PKCZ. Dephosphorylation by calcineurin seems to lead to receptor desensitization and phosphorylation by CAMKII recovers activity.
  • Cellular localization
    Cell junction > synapse > postsynaptic cell membrane. Cell projection > dendritic spine membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Capsaicin receptor antibody
    • DKFZp434K0220 antibody
    • osm 9 like TRP channel 1 antibody
    • Osm-9-like TRP channel 1 antibody
    • OTRPC1 antibody
    • Transient receptor potential cation channel subfamily V member 1 antibody
    • TRPV 1 antibody
    • Trpv1 antibody
    • TRPV1_HUMAN antibody
    • Vanilloid receptor 1 antibody
    • Vanilloid receptor subtype 1 antibody
    • VR 1 antibody
    • VR1 antibody
    see all

Images

  • Immunohistochemical (Frozen) analysis of mouse rectum tissue section labeling VR1 with ab6166 at 1/50 diilution. The tissue was fixed with Paraformaldehyde and permeabilized with 5% acetone and 0.1% Triton. ab150077 at 1/200 was used as the secondary antibody.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat dorsal root ganglia labelling VR1 with ab6166. DAB staining (1/2000). Magnification: 40X.

References

This product has been referenced in:
  • Dos Anjos-Garcia T & Coimbra NC Opposing roles of dorsomedial hypothalamic CB1 and TRPV1 receptors in anandamide signaling during the panic-like response elicited in mice by Brazilian rainbow Boidae snakes. Psychopharmacology (Berl) N/A:N/A (2019). Read more (PubMed: 30694375) »
  • Zhong B  et al. TRPV1 protects renal ischemia-reperfusion injury in diet-induced obese mice by enhancing CGRP release and increasing renal blood flow. PeerJ 7:e6505 (2019). Read more (PubMed: 30834186) »
See all 4 Publications for this product

Customer reviews and Q&As

1-5 of 5 Q&A

Question
Answer

I'm happy to help! Please let me know if you need anything further.

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Answer

Thanks for the additional information.

I have located the order on our system and have arranged for a replacement vial of ab6166 to be included in Abcam's next shipment to you.

For your records I have found the Abcam order reference number for that order was xxxx.

Please let me know if you need anything further.

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Answer

Thank you for your enquiry.

I am sorry to hear of your customer's troubles using ab6166. I have read the attached complaint report and it does appear the most recent vial of ab6166 is not working as expected. I would be happy to offer a replacement or credit.

I have been unsuccessful in locating the order based on the PO. Please double check this number. Thanks!

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Answer

Thank you for your enquiry and interest. This antibody is not purified and as the datasheet indicates we sell it as whole antiserum. ab6166 is a polyclonal antibody and there is batch-to-batch variation; some bleed may contain higher levels of red blood cells/or breakdown product of hemoglobulins than others. If you need any further assistance in the future, please do not hesitate to contact me.

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Question
Answer

This antibody was raised against the intra-cellular C-terminal.

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