Overview

  • Product name

    Anti-VR1 antibody [BS397] - C-terminal
    See all VR1 primary antibodies
  • Description

    Mouse monoclonal [BS397] to VR1 - C-terminal
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-Fr, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat
    Predicted to work with: Guinea pig
  • Immunogen

    Synthetic peptide corresponding to Rat VR1 aa 819-838 (C terminal) (Cysteine residue).
    Sequence:

    GSLKPEDAEVFKDSMVPGEK


    Database link: O35433

  • Positive control

    • WB: PC-12 cell lysates; Mouse brain homogenate; forskolin and NGF stimulated 50B11 hybrid mouse and rat DRG cell lysates; NGF-stimulated PC-12 cell lysates. IHC-Fr: Mouse dorsal root ganglia. ICC/IF: C6 cells. Flow Cytometry: PC-12 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab203103 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 95 kDa.
IHC-Fr Use a concentration of 1 - 10 µg/ml.

Pre-absorption with immunogen obliterates positive staining. Frozen or PEG embedded tissues tested.

ICC/IF Use a concentration of 1 - 2 µg/ml.

Requires permeabilization step due to antigen epitope is intracellular

Flow Cyt Use 2µg for 106 cells.

Target

  • Function

    Receptor-activated non-selective calcium permeant cation channel involved in detection of noxious chemical and thermal stimuli. Seems to mediate proton influx and may be involved in intracellular acidosis in nociceptive neurons. May be involved in mediation of inflammatory pain and hyperalgesia. Sensitized by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases, which involves PKC isozymes and PCL. Acts as ionotropic endocannabinoid receptor with central neuromodulatory effects. Triggers a form of long-term depression (TRPV1-LTD) mediated by the endocannabinoid anandamine in the hippocampus and nucleus accubens by affecting AMPA receptors endocytosis.
  • Tissue specificity

    Widely expressed at low levels. Expression is elevated in dorsal root ganglia. In skin, expressed in cutaneous sensory nerve fibers, mast cells, epidermal keratinocytes, dermal blood vessels, the inner root sheet and the infundibulum of hair follicles, differentiated sebocytes, sweat gland ducts, and the secretory portion of eccrine sweat glands (at protein level).
  • Sequence similarities

    Belongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV1 sub-subfamily.
    Contains 6 ANK repeats.
  • Domain

    The association domain (AD) is necessary for self-association.
  • Post-translational
    modifications

    Phosphorylation by PKA reverses capsaicin-induced dephosphorylation at multiple sites, probably including Ser-117 as a major phosphorylation site. Phoshphorylation by CAMKII seems to regulate binding to vanilloids. Phosphorylated and modulated by PKCM and probably PKCZ. Dephosphorylation by calcineurin seems to lead to receptor desensitization and phosphorylation by CAMKII recovers activity.
  • Cellular localization

    Cell junction > synapse > postsynaptic cell membrane. Cell projection > dendritic spine membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Capsaicin receptor antibody
    • DKFZp434K0220 antibody
    • osm 9 like TRP channel 1 antibody
    • Osm-9-like TRP channel 1 antibody
    • OTRPC1 antibody
    • Transient receptor potential cation channel subfamily V member 1 antibody
    • TRPV 1 antibody
    • Trpv1 antibody
    • TRPV1_HUMAN antibody
    • Vanilloid receptor 1 antibody
    • Vanilloid receptor subtype 1 antibody
    • VR 1 antibody
    • VR1 antibody
    see all

Images

  • Immunohistochemical analysis of mouse dorsal root ganglia labelling VR1 with ab203103 at 10 µg/mL. Visualized with anti-mouse-Cy3 conjugate (red).

     

  • Lane 1 : Anti-VR1 antibody [BS397] - C-terminal (ab203103) at 2 µg/ml (overnight)
    Lane 2 : Anti-VR1 antibody [BS397] - C-terminal (ab203103) at 2 µg/ml ((pre-absorbed))

    All lanes : Rat PC-12 (Rat adrenal gland pheochromocytoma cell line) cell lysate

    Lysates/proteins at 80 µg per lane.

    Secondary
    All lanes : Anti-mouse-HRP at 1/6000 dilution

    Developed using the ECL technique.

    Predicted band size: 95 kDa



    SDS-PAGE: denatured and reduced

  • ab203103 staining VR1 in C6 (Rat glial tumor cell line) cells by ICC/IF (Immunocytochemistry/immunofluorescence).

    Cells were fixed with 4% formaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% horse serum. Samples were incubated with primary antibody (2 μg/ml, panel B) for 1 hour. A CF488A-conjugated Donkey anti-mouse polyclonal was used as the secondary antibody (5 μg/ml). Nuclei were stained with Hoechst dye (panel A) and panel C shows the merged image.

  • Analysis of TRPV1 expression in PC-12 (Rat adrenal gland pheochromocytoma cell line) cell line by Flow Cytometry (black solid).

    Cells were fixed and permeabilized in absolute methanol (10 minutes in ice) and 0.1% Tween-20 in PBS, then blocked with 1% BSA. Incubated with primary antibody for 30 minutes at RT. Secondary antibody used is a Goat anti-mouse PE labeled secondary antibody (1:100 dilution), 20 minutes in dark at room temperature.

    Negative control: Non-specific Control IgG, clone X63 (black dashed).

  • All lanes : Anti-VR1 antibody [BS397] - C-terminal (ab203103) at 1 µg/ml

    Lane 1 : Control
    Lane 2 : Forskolin stimulated 50B11 hybrid mouse x rat DRG cell lysate
    Lane 3 : Forskolin and NGF stimulated 50B11 hybrid mouse x rat DRG cell lysate
    Lane 4 : NGF-stimulated PC-12 (Rat glial tumor cell line) cell lysate (NGF control)

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 95 kDa



    (denatured and reduced)

  • Lane 1 : Anti-VR1 antibody [BS397] - C-terminal (ab203103) at 2 µg/ml
    Lane 2 : Anti-VR1 antibody [BS397] - C-terminal (ab203103) at 1 µg/ml
    Lane 3 : Anti-VR1 antibody [BS397] - C-terminal (ab203103) at 2 µg/ml (pre-absorbed)

    All lanes : Mouse brain homogenate

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : Anti-mouse-HRP at 1/6000 dilution

    Developed using the ECL technique.

    Predicted band size: 95 kDa



    SDS-PAGE: denatured and reduced. ab203103 detects bands at 90-100 kDa and 180-200 kDa corresponding to VR1 monomer and dimer.

References

This product has been referenced in:

  • Wang Y  et al. TRPV1 SUMOylation regulates nociceptive signaling in models of inflammatory pain. Nat Commun 9:1529 (2018). Read more (PubMed: 29670121) »
  • Liu C  et al. Long Non-coding RNA BC168687 is Involved in TRPV1-mediated Diabetic Neuropathic Pain in Rats. Neuroscience 374:214-222 (2018). WB . Read more (PubMed: 29421435) »
See all 5 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab203103.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up