Overview

  • Product name

  • Description

    Rabbit polyclonal to VRK1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rhesus monkey
  • Positive control

    • IHC-P: Human liver tissue. WB: HeLa whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab228725 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 45 kDa.

Target

  • Function

    Serine/threonine kinase that phosphorylates 'Thr-18' of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2.
  • Tissue specificity

    Widely expressed. Highly expressed in fetal liver, testis and thymus.
  • Involvement in disease

    Defects in VRK1 are the cause of pontocerebellar hypoplasia type 1 (PCH1) [MIM:607596]; also called pontocerebellar hypoplasia with infantile spinal muscular atrophy or pontocerebellar hypoplasia with anterior horn cell disease. PCH1 is characterized by an abnormally small cerebellum and brainstem, central and peripheral motor dysfunction from birth, gliosis and anterior horn cell degeneration resembling infantile spinal muscular atrophy (SMA).
  • Sequence similarities

    Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. VRK subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Autophosphorylated at various serine and threonine residues. Autophosphorylation does not impair its ability to phosphorylate p53/TP53.
  • Cellular localization

    Nucleus. Dispersed throughout the cell but not located on mitotic spindle or chromatids during mitosis.
  • Information by UniProt
  • Database links

  • Alternative names

    • MGC117401 antibody
    • MGC138280 antibody
    • MGC142070 antibody
    • PCH1 antibody
    • PCH1A antibody
    • Serine/threonine protein kinase VRK1 antibody
    • Serine/threonine-protein kinase VRK1 antibody
    • Vaccinia related kinase 1 antibody
    • Vaccinia virus B1R related kinase 1 antibody
    • Vaccinia-related kinase 1 antibody
    • VRK1 antibody
    • VRK1_HUMAN antibody
    see all

Images

  • Paraffin-embedded human liver tissue stained for VRK1 using ab228725 at 1/500 dilution in immunohistochemical analysis.

  • Anti-VRK1 antibody (ab228725) at 1/1000 dilution + A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg

    Predicted band size: 45 kDa



    10% SDS-PAGE gel.

References

ab228725 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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