Overview

  • Product name

  • Description

    Rabbit polyclonal to VRK1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human VRK1 aa 1-50. The exact sequence is proprietary.
    Database link: Q99986

  • Positive control

    • IP: HeLa whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab241106 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    Serine/threonine kinase that phosphorylates 'Thr-18' of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2.
  • Tissue specificity

    Widely expressed. Highly expressed in fetal liver, testis and thymus.
  • Involvement in disease

    Defects in VRK1 are the cause of pontocerebellar hypoplasia type 1 (PCH1) [MIM:607596]; also called pontocerebellar hypoplasia with infantile spinal muscular atrophy or pontocerebellar hypoplasia with anterior horn cell disease. PCH1 is characterized by an abnormally small cerebellum and brainstem, central and peripheral motor dysfunction from birth, gliosis and anterior horn cell degeneration resembling infantile spinal muscular atrophy (SMA).
  • Sequence similarities

    Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. VRK subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Autophosphorylated at various serine and threonine residues. Autophosphorylation does not impair its ability to phosphorylate p53/TP53.
  • Cellular localization

    Nucleus. Dispersed throughout the cell but not located on mitotic spindle or chromatids during mitosis.
  • Information by UniProt
  • Database links

  • Alternative names

    • MGC117401 antibody
    • MGC138280 antibody
    • MGC142070 antibody
    • PCH1 antibody
    • PCH1A antibody
    • Serine/threonine protein kinase VRK1 antibody
    • Serine/threonine-protein kinase VRK1 antibody
    • Vaccinia related kinase 1 antibody
    • Vaccinia virus B1R related kinase 1 antibody
    • Vaccinia-related kinase 1 antibody
    • VRK1 antibody
    • VRK1_HUMAN antibody
    see all

Images

  • VRK1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241106 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab241106 at 1 µg/ml.

    Lane 1: ab241106 IP in HeLa whole cell lysate.
    Lane 2: Control IgG IP in HeLa whole cell lysate.

    Detection: Chemiluminescence with exposure time of 10 seconds.

    Lysates prepared in NETN lysis buffer.

References

ab241106 has not yet been referenced specifically in any publications.

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