Overview

  • Product name

    Anti-VRK1 antibody [EPR10623(B)]
    See all VRK1 primary antibodies
  • Description

    Rabbit monoclonal [EPR10623(B)] to VRK1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human VRK1. The exact sequence is proprietary.

  • Positive control

    • MCF7, HeLa, 293T, Jurkat whole cell lysate (ab7899); Human colon and lung adenocarcinoma tissues; 293T cells
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab151706 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 45 kDa.
IHC-P 1/50 - 1/100.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Serine/threonine kinase that phosphorylates 'Thr-18' of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2.
    • Tissue specificity

      Widely expressed. Highly expressed in fetal liver, testis and thymus.
    • Involvement in disease

      Defects in VRK1 are the cause of pontocerebellar hypoplasia type 1 (PCH1) [MIM:607596]; also called pontocerebellar hypoplasia with infantile spinal muscular atrophy or pontocerebellar hypoplasia with anterior horn cell disease. PCH1 is characterized by an abnormally small cerebellum and brainstem, central and peripheral motor dysfunction from birth, gliosis and anterior horn cell degeneration resembling infantile spinal muscular atrophy (SMA).
    • Sequence similarities

      Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. VRK subfamily.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications

      Autophosphorylated at various serine and threonine residues. Autophosphorylation does not impair its ability to phosphorylate p53/TP53.
    • Cellular localization

      Nucleus. Dispersed throughout the cell but not located on mitotic spindle or chromatids during mitosis.
    • Information by UniProt
    • Database links

    • Alternative names

      • MGC117401 antibody
      • MGC138280 antibody
      • MGC142070 antibody
      • PCH1 antibody
      • PCH1A antibody
      • Serine/threonine protein kinase VRK1 antibody
      • Serine/threonine-protein kinase VRK1 antibody
      • Vaccinia related kinase 1 antibody
      • Vaccinia virus B1R related kinase 1 antibody
      • Vaccinia-related kinase 1 antibody
      • VRK1 antibody
      • VRK1_HUMAN antibody
      see all

    Images

    • All lanes : Anti-VRK1 antibody [EPR10623(B)] (ab151706) at 1/1000 dilution

      Lane 1 : MCF7 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 45 kDa

    • Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling VRK1 with ab151706 at 1/50 dilution.
    • Immunohistochemical analysis of paraffin-embedded Human lung adenocarcinoma tissue labelling VRK1 with ab151706 at 1/50 dilution.
    • Flow cytometric analysis of permeabilized 293T cells labeling VRK1 with ab151706 at 1/10 dilution (red) compared to a Rabbit IgG negative control (green).
    • Immunohistochemical analysis of paraffin embedded normal Human liver tissue using ab151706 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human Cervical carcinoma tissue using ab151706 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded normal Human Normal testis tissue using ab151706 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded normal Human uterus tissue using ab151706 showing +ve staining.

    References

    ab151706 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab151706.
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