Overview

  • Product name
  • Description
    Rabbit polyclonal to VRL1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Dot blot, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Synthetic peptide:

    CKNSASEEDHLPLQVLQSP

    (Rat) conjugated to KLH by a Glutaraldehyde linker.

  • General notes
    This antibody is excellent for immunohistochemistry and western blot. VRL 1 is also known as Vanilloid Receptor Like protein-1.

Properties

Applications

Our Abpromise guarantee covers the use of ab6183 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot Use at an assay dependent concentration.
IHC-Fr Use a concentration of 0.5 - 1 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml.

Target

  • Function
    Calcium-permeable, non-selective cation channel with an outward rectification. Seems to be regulated, at least in part, by IGF-I, PDGF and neuropeptide head activator. May transduce physical stimuli in mast cells. Activated by temperatures higher than 52 degrees Celsius; is not activated by vanilloids and acidic pH.
  • Sequence similarities
    Belongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV2 sub-subfamily.
    Contains 6 ANK repeats.
  • Post-translational
    modifications
    N-glycosylated.
    Phosphorylated by PKA.
  • Cellular localization
    Cell membrane. Cytoplasm. Melanosome. Translocates from the cytoplasm to the plasma membrane upon ligand stimulation (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • MGC12549 antibody
    • Osm 9 like TRP channel 2 antibody
    • Osm-9-like TRP channel 2 antibody
    • OTRPC2 antibody
    • Sac2b antibody
    • Stretch activated channel 2B antibody
    • Transient receptor potential cation channel subfamily V member 2 antibody
    • TRPV2 antibody
    • TRPV2_HUMAN antibody
    • Vanilloid receptor like protein 1 antibody
    • Vanilloid receptor-like protein 1 antibody
    • VRL 1 antibody
    • VRL antibody
    • VRL-1 antibody
    • VRL1 antibody
    see all

References

This product has been referenced in:
  • Shimohira D  et al. TRPV2 expression in rat oral mucosa. Histochem Cell Biol 132:423-33 (2009). IHC-FoFr ; Rat . Read more (PubMed: 19579031) »
  • Yamamoto Y  et al. Immunohistochemical colocalization of TREK-1, TREK-2 and TRAAK with TRP channels in the trigeminal ganglion cells. Neurosci Lett 454:129-33 (2009). IHC-Fr ; Rat . Read more (PubMed: 19429069) »
See all 3 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. I have read your technical questionaire and I have a few comments. We have details of this antibody applied by IHC from customer feedback, but not western blotting. You are obtaining an alarming number of extraneous bands. I would like to recommend that in order to obtain more specific results that you use 3% BSA as a blocking agent. This is routinely employed by our lab team. I would also like to recommend that you increase the percentage of Tween 20 to 0.1% in an effort to address the non-specific nature of this antibody. I would also like to recommend that you perform an overnight block of your membrane using this agent. If this does not improve your results please get back in touch with me and I am happy to provide you with a replacement antibody or credit against your purchase provided that you purchased this antibody within the past 90 days.

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Question
Answer

Thank you for your enquiry. The concentration of this antibody is 1 mg/ml. Please contact us again if you have any additional questions.

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Question
Answer

The concentration is 1 mg/ml.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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