Anti-VRTN antibody (ab121138)
Key features and details
- Rabbit polyclonal to VRTN
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-VRTN antibody -
Description
Rabbit polyclonal to VRTN -
Host species
Rabbit -
Tested Applications & Species
Application Species ICC/IF HumanIHC-P Human -
Immunogen
Recombinant fragment corresponding to amino acids 151-286 of Human VRTN (Q9H8Y1).
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Positive control
- Human small intestine tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab121138 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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ICC/IF |
Human
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IHC-P |
Human
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Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment |
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.
|
Notes |
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ICC/IF
Use a concentration of 1 - 4 µg/ml. Recommend PFA Fixation and Triton X-100 treatment |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval. |
Target
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Sequence similarities
Belongs to the vertnin family. - Information by UniProt
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Database links
- Entrez Gene: 55237 Human
- SwissProt: Q9H8Y1 Human
- Unigene: 578167 Human
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Alternative names
- C14orf115 antibody
- Vertnin antibody
- vrtn antibody
- VRTN_HUMAN antibody
Images
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Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli. Recommended concentration of ab121138 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VRTN antibody (ab121138)Immunohistochemical staining of paraffin embedded Human small intestine tissue with anti-VRTN antibody (ab121138; 1:75 dilution)showing nuclear and cytoplasmic positivity in glandular cells.
Protocols
Datasheets and documents
References (0)
ab121138 has not yet been referenced specifically in any publications.