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    vrtn-antibody-ab121138.pdf

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Epigenetics and Nuclear Signaling Chromatin Binding Proteins DNA / RNA binding
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Anti-VRTN antibody (ab121138)

  • Datasheet
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Immunocytochemistry/ Immunofluorescence - Anti-VRTN antibody (ab121138)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VRTN antibody (ab121138)

Key features and details

  • Rabbit polyclonal to VRTN
  • Suitable for: ICC/IF, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-VRTN antibody
  • Description

    Rabbit polyclonal to VRTN
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment corresponding to amino acids 151-286 of Human VRTN (Q9H8Y1).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Human small intestine tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • DNA / RNA binding

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab121138 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IHC-P
Human
Application Abreviews Notes
ICC/IF
Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.
Notes
ICC/IF
Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.

Target

  • Sequence similarities

    Belongs to the vertnin family.
  • Target information above from: UniProt accession Q9H8Y1 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 55237 Human
    • SwissProt: Q9H8Y1 Human
    • Unigene: 578167 Human
    • Alternative names

      • C14orf115 antibody
      • Vertnin antibody
      • vrtn antibody
      • VRTN_HUMAN antibody

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-VRTN antibody (ab121138)
      Immunocytochemistry/ Immunofluorescence - Anti-VRTN antibody (ab121138)
      Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli. Recommended concentration of ab121138 1-4 µg/ml. Cells treated with PFA/Triton X-100.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VRTN antibody (ab121138)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VRTN antibody (ab121138)
      Immunohistochemical staining of paraffin embedded Human small intestine tissue with anti-VRTN antibody (ab121138; 1:75 dilution)showing nuclear and cytoplasmic positivity in glandular cells.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab121138? Please let us know so that we can cite the reference in this datasheet.

    ab121138 has not yet been referenced specifically in any publications.

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