Anti-VRTN antibody (ab121138)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-VRTN antibody
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DescriptionRabbit polyclonal to VRTN
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Host speciesRabbit
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Tested applicationsSuitable for: ICC/IF, IHC-Pmore details
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Species reactivityReacts with: Human
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Immunogen
Recombinant fragment corresponding to amino acids 151-286 of Human VRTN (Q9H8Y1).
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Positive control
- Human small intestine tissue.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab121138 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 1 - 4 µg/ml. Recommend PFA Fixation and Triton X-100 treatment |
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| IHC-P | 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval. |
Target
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Sequence similaritiesBelongs to the vertnin family.
- Information by UniProt
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Database links
- Entrez Gene: 55237 Human
- SwissProt: Q9H8Y1 Human
- Unigene: 578167 Human
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Alternative names
- C14orf115 antibody
- Vertnin antibody
- vrtn antibody
- VRTN_HUMAN antibody
Images
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Immunocytochemistry/ Immunofluorescence - Anti-VRTN antibody (ab121138)Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli. Recommended concentration of ab121138 1-4 µg/ml. Cells treated with PFA/Triton X-100. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VRTN antibody (ab121138)Immunohistochemical staining of paraffin embedded Human small intestine tissue with anti-VRTN antibody (ab121138; 1:75 dilution)showing nuclear and cytoplasmic positivity in glandular cells.
Datasheets and documents
References
ab121138 has not yet been referenced specifically in any publications.
