Anti-VRTN antibody (ab121138)

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Overview

  • Product name

    Anti-VRTN antibody

  • Description

    Rabbit polyclonal to VRTN

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment corresponding to amino acids 151-286 of Human VRTN (Q9H8Y1).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Human small intestine tissue.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121138 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.

Target

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-VRTN antibody (ab121138)
    Immunocytochemistry/ Immunofluorescence - Anti-VRTN antibody (ab121138)
    Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli. Recommended concentration of ab121138 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VRTN antibody (ab121138)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VRTN antibody (ab121138)
    Immunohistochemical staining of paraffin embedded Human small intestine tissue with anti-VRTN antibody (ab121138; 1:75 dilution)showing nuclear and cytoplasmic positivity in glandular cells.

References

ab121138 has not yet been referenced specifically in any publications.

Publishing research using ab121138? Please let us know so that we can cite the reference in this datasheet.

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