Key features and details
- Rabbit polyclonal to VSIG4
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-VSIG4 antibody
See all VSIG4 primary antibodies
DescriptionRabbit polyclonal to VSIG4
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
- WB: Human lung lysate. IHC-P: Human placenta and lung tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab246869 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 44 kDa.|
FunctionPhagocytic receptor, strong negative regulator of T-cell proliferation and IL2 production. Potent inhibitor of the alternative complement pathway convertases.
Tissue specificityAbundantly expressed in several fetal tissues. In adult tissues, highest expression in lung and placenta. Expressed in resting macrophages.
Sequence similaritiesContains 2 Ig-like (immunoglobulin-like) domains.
- Information by UniProt
- Complement receptor of the immunoglobulin superfamily antibody
- CRIg antibody
- Ig superfamily protein antibody
Paraffin-embedded human lung tissue stained for VSIG4 using ab246869 at 1/200 dilution in immunohistochemical analysis.
Anti-VSIG4 antibody (ab246869) at 0.04 µg/ml + Human lung tissue lysate
Predicted band size: 44 kDa
Western blot analysis labeling VSIG4 with ab246869 at 0.04 ug/ml.
Paraffin-embedded human placenta tissue stained for VSIG4 using ab246869 at 1/200 dilution in immunohistochemical analysis.
Paraffin-embedded human skeletal muscle tissue stained for VSIG4 using ab246869 at 1/200 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab246869 has not yet been referenced specifically in any publications.