Recombinant Anti-VSTM2B antibody [EPR10949] - BSA and Azide free (ab249803)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10949] to VSTM2B - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-VSTM2B antibody [EPR10949] - BSA and Azide free
See all VSTM2B primary antibodies -
Description
Rabbit monoclonal [EPR10949] to VSTM2B - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab249803 is the carrier-free version of ab174299.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR10949 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab249803 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 30 kDa.
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IP |
Use at an assay dependent concentration.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 30 kDa. |
IP
Use at an assay dependent concentration. |
Target
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Relevance
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Membrane; Single-pass type I membrane protein -
Database links
- Entrez Gene: 342865 Human
- Entrez Gene: 58188 Mouse
- Entrez Gene: 361560 Rat
- SwissProt: A6NLU5 Human
- SwissProt: Q9JME9 Mouse
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Alternative names
- V set and transmembrane domain containing 2B antibody
- V-set and transmembrane domain-containing protein 2B antibody
- VSTM2B antibody
Images
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This data was developed using ab174299, the same antibody clone in a different buffer formulation.Western blot analysis on immunoprecipitation pellet from (1) fetal brain lysate or (2) 1X PBS (negative control) using ab174299 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab249803 has not yet been referenced specifically in any publications.