Anti-VSV-G tag antibody (ab1874)

Thank you for your enquiry and your interest in our products.

We have a few antibodies which detect VSV-G and are suitable for Western applications. Some of them are unconjugated so it is essential to apply a compatible secondary antibody for detection:

ab1874: https://www.abcam.com/vsv-g-tag-antibody-ab1874.html

ab27026: https://www.abcam.com/vsv-g-tag-antibody-ab27026.html

ab50549: https://www.abcam.com/vsv-g-tag-antibody-p5d4-ab50549.html

Some others are HRP-labelled so can be applied without any secondary antibodies:

ab3556: https://www.abcam.com/vsv-g-tag-antibody-hrp-ab3556.html

ab49610: https://www.abcam.com/vsv-g-tag-antibody-p5d4-hrp-ab49610.html

Currently, we do not sel lpositive control cell lysate for VSV-G. As long as the cells you are planning to use express the tag sequence: YTDIEMNRLGK; these antibodies should work fine.

The antibody has not been tested in this way so we do not know it it will bind.

We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you decide to test an antibody in an application for which we do not have any information, please let us know how you get on and in return we will forward a GBP10/ USD15/ EUR15 Amazon gift voucher.

Thank you for contacting us and your interest in our products.

As discussed over the phone, I have looked into the antibodies we have which are directed against the VSV-G tag. As you have seen, the reference where ab1874 was used in the staining of frozen rat lung tissue were not used to detect the epitope directly as part of the tissue. It was used to detect and antibody directed against heparan sulfate which contained the tag. The antibody was therefore used more like a secondary antibody.

Unfortunately, we do not have any antibodies where the VSV-G epitope has been detected for IHC as yet. This does not mean that the antibodies would not work in this application, simply that we have not tested it ourselves and would not be able to guarantee its performance.

I am however able to offer you a testing discount if you would like to try one of our antibodies. This offer means that if you purchase an antibody such as the rabbit polyclonal https://www.abcam.com/VSV-G-tag-antibody-ab1874.html or chicken polyclonal https://www.abcam.com/VSV-G-tag-antibody-ab27026.html as normal, test the antibody with your mouse samples in IHC and let us know of the results through an Abreview (no matter whether positive or negative) you would be eligible for the value of the antibody tested off any future purchase with Abcam. More information on this offer can be found from the following link:

https://www.abcam.com/abtrial

Please note that the antibody to be tested must be purchased, tested, the Abreview submitted and the free product claimed within a 4 month period.

If you would be interested in participating in this scheme please do let me know as a discount code needs to be issued prior to the purchase of the antibody to be tested.

I hope this information has been of help. If you require any further information please do not hesitate to contact us again.