- Datasheet
- Specific References (11)
- Protocols
Overview
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Product nameAnti-VSV-G tag antibody
See all VSV-G tag primary antibodies -
DescriptionRabbit polyclonal to VSV-G tag
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Host speciesRabbit
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SpecificityThis product has been assayed by ELISA against 0.1 µg of the immunizing peptide. A 1/8000 to 1/32000 dilution of the antibody is recommended for this assay.
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Tested applicationsSuitable for: ICC, IHC-Fr, WB, IP, ICC/IFmore details
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Immunogen
A synthetic peptide corresponding to aa 501-511 of vesicular stomatitis virus glycoprotein (VSV-G). This vector is a pseudo lentiviral vector. Peptide sequence:
YTDIEMNRLGK
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Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
PurityWhole antiserum
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab1874 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC | 1/100. | |
| IHC-Fr | Use at an assay dependent concentration. PubMed: 15662032 | |
| WB | Use at an assay dependent concentration. | |
| IP | Use at an assay dependent concentration. | |
| ICC/IF | 1/200. |
Target
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RelevanceVesicular stomatitis virus (VSV), an enveloped RNA virus from the Rhabdoviridae family, is released from the plasma membrane of host cells by a process called budding. The glycoprotein (VSV-g) contains a domain in its extracellular membrane proximal stem that appears to be needed for efficient VSV budding.
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Alternative names
- Vesicular stomatitis virus glycoprotein tag antibody
- VSV epitope tag antibody
- VSV tag antibody
see all
Images
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Immunohistochemistry (Frozen sections) - Anti-VSV-G tag antibody (ab1874)Image from Thompson SM et al, J Biol Chem. 2009 Dec 18;284(51):35621-31, Fig 1. DOI 10.1074/jbc.M109.009712ab1874 detecting bound HS scFv antibody in rat lung tissue by Immunohistochemistry (Frozen sections).
Lungs from Sprague-Dawley rats were harvested at embryonic days 17.5 of gestation and fixed with 4% (w/v) paraformaldehyde in phosphate-buffered saline (PBS), pH 7.4. The lungs were washed in PBS, cryoprotected with 20% (w/v) sucrose in PBS, and gelatin-embedded (7.5% (w/v) gelatin, 15% (w/v) sucrose in PBS) before being covered in Cryo-M-Bed and snap frozen at -40 °C. The lung sections were cut at 8 µm and mounted onto chrome alum gel slides for storage at -40 °C. Tissue sections were blocked with 10% (v/v) goat serum in PBS and incubated overnight at 4 °C with HS scFv antibody, diluted 1/5 in 1% (v/v) goat serum in PBS. Bound antibody was detected with ab1874 diluted 1/200 in 1% (v/v) goat serum in PBS, followed by fluorescein isothiocyanate-conjugated goat anti-rabbit IgG.
aw, airway; me, mesenchyme; bm, basement membrane.
Protocols
References
This product has been referenced in:
- Dutta P et al. A novel modelling mechanism of PAEL receptor and GABARAPL2 interaction involved in Parkinson's disease. Neurosci Lett 673:12-18 (2018). ICC/IF . Read more (PubMed: 29496607) »
- Havekes R et al. Compartmentalized PDE4A5 Signaling Impairs Hippocampal Synaptic Plasticity and Long-Term Memory. J Neurosci 36:8936-46 (2016). WB, IHC, ICC/IF . Read more (PubMed: 27559174) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"