Anti-VSV-G tag antibody (ab1874)

Reviews (2)Q&A (4)References (12)

Overview

  • Product name
    Anti-VSV-G tag antibody
    See all VSV-G tag primary antibodies
  • Description
    Rabbit polyclonal to VSV-G tag
  • Host species
    Rabbit
  • Specificity
    This product has been assayed by ELISA against 0.1 µg of the immunizing peptide. A 1/8000 to 1/32000 dilution of the antibody is recommended for this assay.
  • Tested applications
    Suitable for: ICC, IHC-Fr, WB, IP, ICC/IFmore details
  • Immunogen

    A synthetic peptide corresponding to aa 501-511 of vesicular stomatitis virus glycoprotein (VSV-G). This vector is a pseudo lentiviral vector. Peptide sequence:

    YTDIEMNRLGK

    .

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Purity
    Whole antiserum
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab1874 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/100.
IHC-Fr Use at an assay dependent concentration. PubMed: 15662032
WB Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
ICC/IF 1/200.

Target

  • Relevance
    Vesicular stomatitis virus (VSV), an enveloped RNA virus from the Rhabdoviridae family, is released from the plasma membrane of host cells by a process called budding. The glycoprotein (VSV-g) contains a domain in its extracellular membrane proximal stem that appears to be needed for efficient VSV budding.
  • Alternative names
    • Vesicular stomatitis virus glycoprotein tag antibody
    • VSV epitope tag antibody
    • VSV tag antibody
    • VSV-G epitope tag antibody
    • VSVG antibody
    • vsvg tag antibody
    see all

Images

  • Immunohistochemistry (Frozen sections) - Anti-VSV-G tag antibody (ab1874)
    Immunohistochemistry (Frozen sections) - Anti-VSV-G tag antibody (ab1874)Image from Thompson SM et al, J Biol Chem. 2009 Dec 18;284(51):35621-31, Fig 1. DOI 10.1074/jbc.M109.009712
    ab1874 detecting bound HS scFv antibody in rat lung tissue by Immunohistochemistry (Frozen sections).
    Lungs from Sprague-Dawley rats were harvested at embryonic days 17.5 of gestation and fixed with 4% (w/v) paraformaldehyde in phosphate-buffered saline (PBS), pH 7.4. The lungs were washed in PBS, cryoprotected with 20% (w/v) sucrose in PBS, and gelatin-embedded (7.5% (w/v) gelatin, 15% (w/v) sucrose in PBS) before being covered in Cryo-M-Bed and snap frozen at -40 °C. The lung sections were cut at 8 µm and mounted onto chrome alum gel slides for storage at -40 °C. Tissue sections were blocked with 10% (v/v) goat serum in PBS and incubated overnight at 4 °C with HS scFv antibody, diluted 1/5 in 1% (v/v) goat serum in PBS. Bound antibody was detected with ab1874 diluted 1/200 in 1% (v/v) goat serum in PBS, followed by fluorescein isothiocyanate-conjugated goat anti-rabbit IgG.

    aw, airway; me, mesenchyme; bm, basement membrane.

References

This product has been referenced in:
  • Dutta P  et al. A novel modelling mechanism of PAEL receptor and GABARAPL2 interaction involved in Parkinson's disease. Neurosci Lett 673:12-18 (2018). ICC/IF . Read more (PubMed: 29496607) »
  • Pužar Dominkuš P  et al. Nef is secreted in exosomes from Nef.GFP-expressing and HIV-1-infected human astrocytes. J Neurovirol 23:713-724 (2017). Read more (PubMed: 28762184) »
See all 12 Publications for this product

Publishing research using ab1874? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-6 of 6 Abreviews or Q&A

Question

Hello,



I am looking for an antibody for detecting whether my cells are expressing VSV-G (Western blot). I have found several ab on-line, but do you also have cell lysate as a positive control?



Best regards

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Answer

Thank you for your enquiry and your interest in our products.

We have a few antibodies which detect VSV-G and are suitable for Western applications. Some of them are unconjugated so it is essential to apply a compatible secondary antibody for detection:

ab1874: https://www.abcam.com/vsv-g-tag-antibody-ab1874.html

ab27026: https://www.abcam.com/vsv-g-tag-antibody-ab27026.html

ab50549: https://www.abcam.com/vsv-g-tag-antibody-p5d4-ab50549.html

Some others are HRP-labelled so can be applied without any secondary antibodies:

ab3556: https://www.abcam.com/vsv-g-tag-antibody-hrp-ab3556.html

ab49610: https://www.abcam.com/vsv-g-tag-antibody-p5d4-hrp-ab49610.html

Currently, we do not sel lpositive control cell lysate for VSV-G. As long as the cells you are planning to use express the tag sequence: YTDIEMNRLGK; these antibodies should work fine.

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Immunocytochemistry abreview for Anti-VSV-G tag antibody

 Below Average
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
abreview image
Application
Immunocytochemistry
Sample
Saccharomyces cerevisiae Cell
Fixative
Formaldehyde
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1%
Read More

Mr. Chesneau Laurent

Verified customer

Submitted Sep 19 2005

Western blot abreview for Anti-VSV-G tag antibody

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Saccharomyces cerevisiae Cell lysate - whole cell
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5%
Read More

Mr. Chesneau Laurent

Verified customer

Submitted Sep 02 2005

Question

I would like to know if this antibody (AB1874, VSV-G tag) is able to bind to recombinant lentiviral vectors pseudotyped with the VSV-G enveloppe.

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Answer

The antibody has not been tested in this way so we do not know it it will bind.

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Question

I would like to know if this antibody (AB1874, VSV-G tag) is able to bind to recombinant lentiviral vectors pseudotyped with the VSV-G enveloppe.

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Answer

We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you decide to test an antibody in an application for which we do not have any information, please let us know how you get on and in return we will forward a GBP10/ USD15/ EUR15 Amazon gift voucher.

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Question

Phone call requesting information on antibodies directed against the VSV-G tag for use in IHC.

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Answer

Thank you for contacting us and your interest in our products.

As discussed over the phone, I have looked into the antibodies we have which are directed against the VSV-G tag. As you have seen, the reference where ab1874 was used in the staining of frozen rat lung tissue were not used to detect the epitope directly as part of the tissue. It was used to detect and antibody directed against heparan sulfate which contained the tag. The antibody was therefore used more like a secondary antibody.

Unfortunately, we do not have any antibodies where the VSV-G epitope has been detected for IHC as yet. This does not mean that the antibodies would not work in this application, simply that we have not tested it ourselves and would not be able to guarantee its performance.

I am however able to offer you a testing discount if you would like to try one of our antibodies. This offer means that if you purchase an antibody such as the rabbit polyclonal https://www.abcam.com/VSV-G-tag-antibody-ab1874.html or chicken polyclonal https://www.abcam.com/VSV-G-tag-antibody-ab27026.html as normal, test the antibody with your mouse samples in IHC and let us know of the results through an Abreview (no matter whether positive or negative) you would be eligible for the value of the antibody tested off any future purchase with Abcam. More information on this offer can be found from the following link:

https://www.abcam.com/abtrial

Please note that the antibody to be tested must be purchased, tested, the Abreview submitted and the free product claimed within a 4 month period.

If you would be interested in participating in this scheme please do let me know as a discount code needs to be issued prior to the purchase of the antibody to be tested.

I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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