Product nameAnti-VSV-G tag antibody
See all VSV-G tag primary antibodies
DescriptionRabbit polyclonal to VSV-G tag
SpecificityThis product has been assayed by ELISA against 0.1 µg of the immunizing peptide. A 1/8000 to 1/32000 dilution of the antibody is recommended for this assay.
Tested applicationsSuitable for: ICC, IHC-Fr, WB, IP, ICC/IFmore details
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
Our Abpromise guarantee covers the use of ab1874 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration. PubMed: 15662032|
|WB||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
RelevanceVesicular stomatitis virus (VSV), an enveloped RNA virus from the Rhabdoviridae family, is released from the plasma membrane of host cells by a process called budding. The glycoprotein (VSV-g) contains a domain in its extracellular membrane proximal stem that appears to be needed for efficient VSV budding.
- Vesicular stomatitis virus glycoprotein tag antibody
- VSV epitope tag antibody
- VSV tag antibody
ab1874 detecting bound HS scFv antibody in rat lung tissue by Immunohistochemistry (Frozen sections).
Lungs from Sprague-Dawley rats were harvested at embryonic days 17.5 of gestation and fixed with 4% (w/v) paraformaldehyde in phosphate-buffered saline (PBS), pH 7.4. The lungs were washed in PBS, cryoprotected with 20% (w/v) sucrose in PBS, and gelatin-embedded (7.5% (w/v) gelatin, 15% (w/v) sucrose in PBS) before being covered in Cryo-M-Bed and snap frozen at -40 °C. The lung sections were cut at 8 µm and mounted onto chrome alum gel slides for storage at -40 °C. Tissue sections were blocked with 10% (v/v) goat serum in PBS and incubated overnight at 4 °C with HS scFv antibody, diluted 1/5 in 1% (v/v) goat serum in PBS. Bound antibody was detected with ab1874 diluted 1/200 in 1% (v/v) goat serum in PBS, followed by fluorescein isothiocyanate-conjugated goat anti-rabbit IgG.
aw, airway; me, mesenchyme; bm, basement membrane.
This product has been referenced in:
- Dutta P et al. A novel modelling mechanism of PAEL receptor and GABARAPL2 interaction involved in Parkinson's disease. Neurosci Lett 673:12-18 (2018). ICC/IF . Read more (PubMed: 29496607) »
- Pužar Dominkuš P et al. Nef is secreted in exosomes from Nef.GFP-expressing and HIV-1-infected human astrocytes. J Neurovirol 23:713-724 (2017). Read more (PubMed: 28762184) »