Product nameAnti-VSV-G tag antibody
See all VSV-G tag primary antibodies
DescriptionChicken polyclonal to VSV-G tag
SpecificityThis antibody recognises VSV-G tag.
Tested applicationsSuitable for: ICC/IF, IP, WB, ELISAmore details
Synthetic peptide: YTDIEMNRLGK, conjugated to KLH
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.1% Sodium Azide.
Constituents: PBS, pH 7.2.
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThis antibody was immunoaffinity purified off an antigen containing immunoabsorbant.
Our Abpromise guarantee covers the use of ab27026 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent dilution.|
|IP||Use at an assay dependent dilution.|
|WB||1/2000 - 1/10000.|
|ELISA||1/200 - 1/500.|
RelevanceVesicular stomatitis virus (VSV), an enveloped RNA virus from the Rhabdoviridae family, is released from the plasma membrane of host cells by a process called budding. The glycoprotein (VSV-g) contains a domain in its extracellular membrane proximal stem that appears to be needed for efficient VSV budding.
- Vesicular stomatitis virus glycoprotein tag antibody
- VSV epitope tag antibody
- VSV tag antibody
ab27026 staining VSV-G tag in VSV-G-GFP overexpressing Hela cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in formaldehyde, permeabilized in 0.1% saponin and blocked in 20mM glycine in PBS for 20 minutes at 23°C. A Alexa Fluor® 568 conjugated goat polyclonal to chicken was used, undiluted as secondary. Red color repersents staining with ab27026.
This product has been referenced in:
- Lopez CA et al. Label-free multiplexed virus detection using spectral reflectance imaging. Biosens Bioelectron 26:3432-7 (2011). Read more (PubMed: 21342761) »
- Salikhova A et al. Vascular endothelial growth factor and semaphorin induce neuropilin-1 endocytosis via separate pathways. Circ Res 103:e71-9 (2008). ICC/IF . Read more (PubMed: 18723443) »