Anti-VSV-G tag antibody (ab83196)

Rabbit polyclonal VSV-G tag antibody. Validated in WB. Independently reviewed in 3 review(s). Immunogen corresponding to full length protein.

Overview

  • Product name

  • Description

    Rabbit polyclonal to VSV-G tag
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Full length protein corresponding to VSV-G tag aa 1-495.
    Database link: NP_955548

  • General notes

    0.2 µ filtered

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab83196 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Predicted molecular weight: 10 kDa.

Target

  • Relevance

    Vesicular stomatitis virus (VSV), an enveloped RNA virus from the Rhabdoviridae family, is released from the plasma membrane of host cells by a process called budding. The glycoprotein (VSV-g) contains a domain in its extracellular membrane proximal stem that appears to be needed for efficient VSV budding.
  • Alternative names

    • Vesicular stomatitis virus glycoprotein tag antibody
    • VSV epitope tag antibody
    • VSV tag antibody
    • VSV-G epitope tag antibody
    • VSVG antibody
    • vsvg tag antibody
    see all

Images

  • All lanes : Anti-VSV-G tag antibody (ab83196) at 1/1000 dilution

    Lane 1 : Human HEK293 cells transfected with pMD2.G (VSVG encoding construct) whole cell lysate (labelled +VSVG)
    Lane 2 : Human HEK293 mock transfected (labelled -VSVG)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit polyclonal HRP conjugate at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 10 kDa


    Exposure time: 10 seconds

    See Abreview

References

ab83196 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Immunocytochemistry
Sample
Mouse Cultured Cells (4T1)
Permeabilization
Yes - methanol
Specification
4T1
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Formaldehyde

Dr. roberto de Antueno

Verified customer

Submitted Sep 03 2018

Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293 cell line)
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
10 µg
Treatment
Transfected with pMD2.G or mock
Specification
HEK293 cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Sep 28 2015

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEK293 cell line)
Permeabilization
No
Specification
HEK293 cell line
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Sep 28 2015

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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