Application
Immunocytochemistry
Sample
Mouse Cultured Cells (4T1)
Permeabilization
Yes - methanol
Specification
4T1
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Formaldehyde
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 10% FBS in PBS containing 100 mM Glycine
Dilution
1/2000
Secondary antibody
Dilution
1/400
Name
Non-Abcam antibody was used: anti-rabbit-IgG (whole molecule)-alkaline phosphat
Host species: Goat
Clonality: Polyclonal
Conjugation: Alkaline Phosphatase
Host species: Goat
Clonality: Polyclonal
Conjugation: Alkaline Phosphatase
Additional data
Additional Notes
Washing buffer: 0.1% Tween-20 in PBS.
Cells were washed 3x for 10 min at r. temp.
Sec. ab was diluted with the same buffer as the primary ab.
Substrate for A.P: SigmaFast BCIP/NBT
Dr. roberto de Antueno
Verified customer
Submitted Sep 03 2018