Product nameAnti-VSV-G tag antibody (Biotin)
See all VSV-G tag primary antibodies
DescriptionRabbit polyclonal to VSV-G tag (Biotin)
SpecificityThis antibody is directed against VSV-G protein.
Tested applicationsSuitable for: ELISA, WB, ICC/IF, Flow Cytmore details
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Donkey IgG molecule.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.42% Potassium phosphate, 0.87% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab34774 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||1/3000 - 1/12000.|
|WB||1/500 - 1/2000. Predicted molecular weight: 57.5 kDa.|
|ICC/IF||1/200 - 1/1000. We recommend paraformaldehyde fixation with detergent permeabilization of cells infected with VSV or transfected with the wild-type VSV-G protein.|
|Flow Cyt||Use at an assay dependent concentration.
ab200208 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
RelevanceVesicular stomatitis virus (VSV), an enveloped RNA virus from the Rhabdoviridae family, is released from the plasma membrane of host cells by a process called budding. The glycoprotein (VSV-g) contains a domain in its extracellular membrane proximal stem that appears to be needed for efficient VSV budding.
- Vesicular stomatitis virus glycoprotein tag antibody
- VSV epitope tag antibody
- VSV tag antibody
ab34774 staining VSV-G tag in human HeLa cells by Immunocytochemistry/ Immunofluorescence. The cells were paraformaldehyde fixed, permeabilised in 0.025% Triton-X in PBS and then blocked using 5% serum for 1 hour. Samples were then incubated with primary antibody at 1/200 for 16 hours at 4°C. The secondary antibody used was a goat polyclonal conjugated to Alexa Fluor® 488 (green) used undiluted.
Both lanes are ab34774 at a 1/500 dilution plus 20µg of whole cell lysate prepared from HeLa cells transfected with VSV-G. A Streptavidin HRP conjugate was used at a 1/2000 dilution.
Primary antibody incubated for 16 hours at 4°C.Blocking step performed using 5% milk for 1 hour at 23°C.
ab34774 detecting the VSV-G tag in HeLa cells expressing VSV-G by direct ELISA. Samples were blocked with 5% BSA for 1 hour at 23°C and incubated with the primary antibody (1/3000 in blocking buffer) for 16 hours at 4°C. An undiluted streptavidin-alkaline phosphatase conjugate polyclonal was used as the secondary antibody.
This product has been referenced in:
- Thompson A et al. Molecular Characterisation of Small Molecule Agonists Effect on the Human Glucagon Like Peptide-1 Receptor Internalisation. PLoS One 11:e0154229 (2016). Read more (PubMed: 27100083) »
- Wan Z et al. Fangchinoline inhibits human immunodeficiency virus type 1 replication by interfering with gp160 proteolytic processing. PLoS One 7:e39225 (2012). WB . Read more (PubMed: 22720080) »