Overview

  • Product name
    Anti-VSV-G tag antibody (FITC)
    See all VSV-G tag primary antibodies
  • Description
    Goat polyclonal to VSV-G tag (FITC)
  • Host species
    Goat
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Tested applications
    Suitable for: ICC, Flow Cytmore details
  • Species reactivity
    Reacts with: Species independent
  • Immunogen

    Synthetic peptide corresponding to VSV-G tag conjugated to keyhole limpet haemocyanin.
    Sequence:

    YTDIEMNRLGK

  • Positive control
    • Flow Cyt: HUVEC cells.
  • General notes

    F/P ratio is 3 to 7.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C. Store In the Dark.
  • Storage buffer
    pH: 7.20
    Preservative: 0.09% Sodium azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab3863 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/100 - 1/400.
Flow Cyt Use at an assay dependent concentration.

ab37394 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Relevance
    Vesicular stomatitis virus (VSV), an enveloped RNA virus from the Rhabdoviridae family, is released from the plasma membrane of host cells by a process called budding. The glycoprotein (VSV-g) contains a domain in its extracellular membrane proximal stem that appears to be needed for efficient VSV budding.
  • Alternative names
    • Vesicular stomatitis virus glycoprotein tag antibody
    • VSV epitope tag antibody
    • VSV tag antibody
    • VSV-G epitope tag antibody
    • VSVG antibody
    • vsvg tag antibody
    see all

Images

  • The first dot plot (A) corresponds to the sample unstained. The second plot (B) corresponds to the cells stained only with the anti-VSV-g tag antibody FITC, to see the background and the third (C), corresponds to the cells expressing VSV+ stained with anti VSVgFITC antibody.

    For further details please see abreview.

References

ab3863 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Gern geschehen, jederzeit gerne wieder!

Read More
Application
Flow Cytometry
Sample
Human Cell (HUVEC)
Specification
HUVEC
Preparation
Cell harvesting/tissue preparation method: Harvest cells using cell dissociation buffer
Sample buffer: DMEM/10% FBS
Permeabilization
No
Gating Strategy
FITC

Abcam user community

Verified customer

Submitted Oct 27 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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