Overview

  • Product name
    Anti-VSV-G tag antibody (HRP)
    See all VSV-G tag primary antibodies
  • Description
    Rabbit polyclonal to VSV-G tag (HRP)
  • Host species
    Rabbit
  • Conjugation
    HRP
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Immunogen

    Synthetic peptide: YTDIEMNRLGK conjugated to KLH.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.19% HEPES, 0.58% Sodium chloride, 0.2% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Antibodies were immunoaffinity purified using the peptide immobilized on a solid phase.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3556 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000.
ELISA 1/5000 - 1/20000.

Target

  • Relevance
    Vesicular stomatitis virus (VSV), an enveloped RNA virus from the Rhabdoviridae family, is released from the plasma membrane of host cells by a process called budding. The glycoprotein (VSV-g) contains a domain in its extracellular membrane proximal stem that appears to be needed for efficient VSV budding.
  • Alternative names
    • Vesicular stomatitis virus glycoprotein tag antibody
    • VSV epitope tag antibody
    • VSV tag antibody
    • VSV-G epitope tag antibody
    • VSVG antibody
    • vsvg tag antibody
    see all

References

This product has been referenced in:
  • Bellon A  et al. VEGFR2 (KDR/Flk1) signaling mediates axon growth in response to semaphorin 3E in the developing brain. Neuron 66:205-19 (2010). WB . Read more (PubMed: 20434998) »
  • Thyrell L  et al. Interferon alpha-induced apoptosis in tumor cells is mediated through the phosphoinositide 3-kinase/mammalian target of rapamycin signaling pathway. J Biol Chem 279:24152-62 (2004). Read more (PubMed: 15056668) »
See all 2 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for your enquiry and your interest in our products.

We have a few antibodies which detect VSV-G and are suitable for Western applications. Some of them are unconjugated so it is essential to apply a compatible secondary antibody for detection:

ab1874: https://www.abcam.com/vsv-g-tag-antibody-ab1874.html

ab27026: https://www.abcam.com/vsv-g-tag-antibody-ab27026.html

ab50549: https://www.abcam.com/vsv-g-tag-antibody-p5d4-ab50549.html

Some others are HRP-labelled so can be applied without any secondary antibodies:

ab3556: https://www.abcam.com/vsv-g-tag-antibody-hrp-ab3556.html

ab49610: https://www.abcam.com/vsv-g-tag-antibody-p5d4-hrp-ab49610.html

Currently, we do not sel lpositive control cell lysate for VSV-G. As long as the cells you are planning to use express the tag sequence: YTDIEMNRLGK; these antibodies should work fine.

Read More

Question

WESTERN BLOT TECHNICAL ENQUIRY - Wed 11 Feb 2004, 10:42hrs ========================================================== ANTIBODY CODE ab3556 BATCH NUMBER 37614 ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM Non-specific band I immunoprecipitate 2 VSV-G tagged proteins using a mouse monoclonal antibody and want to detect my immunoprecipitatd proteins with the VSV G hrp rabbit polyclonal antibody. I have no problem to detect the first one (so I know that my immunoprecipitation is OK), but the problem is that at the size of my second VSV-G tagged proteins there are plenty of non specific bands, so I detect a band even in my controls where I don't express this second protein. So I cannot prove that I have immunoprecipitated my second protein too. I tried different dilutions of the antibody but this does not solve my problem. Can you indicate me your Western Blot conditions? Furthermore, this antibody reveals the heavy and light chains. I wanted to know if this antody is known to cross-react ? Thank you Nathalie Franck SAMPLE HeLa cell extract incubated with a VSV-G monoclonal antibody (which works well) PRIMARY ANTIBODY VSV-g hrp tested at different dilutions 1/1000-1/5000 SECONDARY ANTIBODY none DETECTION METHOD super signal (Pierce) POSITIVE AND NEGATIVE CONTROLS USED Negative control: Lysates transfected with a control protein also VSV-G tagged. I detect this protein (no contaminating bands at this size (100 kDa). But at the size of my protein of interest, I detect a contaminating band in my negative control ANTIBODY STORAGE CONDITIONS Storage: 4°C SAMPLE PREPARATION Buffer containing 150 mM NaCL, Tris 10 mM, NP40 0,5%, Triton-X-100 1%, EDTA, EGTA, Na3VO4, PMSF, protease inhibitor (Roche) AMOUNT OF PROTEIN LOADED 15-20 µg of total cell lysate or 10 µl of immunoprecipitation product (I use 500 µg for one immunoprecipitation; beads are respuspended in 25 µl of SB2X ELECTROPHORESIS/GEL CONDITIONS SDS-PAGE 12,5% TRANSFER AND BLOCKING CONDITIONS Transfer 45 minutes 300 mA. I block my membran with 5% dry non fat milk in Tris buffered saline containing 0,1% Tween. HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 3 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? Dilution of the antibody and incubation time (overnight or 1h at ambiant temperature

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Answer

Thank you very much for your patience. I am working with the originator of ab3556 to help figure out what may be going on with this particular antibody. We have a few more questions however that will help us out. 1) What exactly does your negative control consist of? 2) How are you isolating the monoclonal antibody/VSV-g tagged protein? 3) Do you isolate by eluting the protein or centrifuging? 4) How do you know it is recognizing the heavy and light chains? 5)Have you tried any inhibition studies?

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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