• Product name
    Anti-VSV-G tag antibody [P5D4]
    See all VSV-G tag primary antibodies
  • Description
    Mouse monoclonal [P5D4] to VSV-G tag
  • Host species
  • Tested applications
    Suitable for: ICC/IF, WB, IP, ICCmore details
  • Species reactivity
    Reacts with VSV-G tag. Not yet tested in other species.
  • Immunogen

    Synthetic peptide corresponding to C terminal amino acids 497-511 of VSV-G tag conjugated to KLH

  • Positive control
    • WB: Whole extract of HEK-293T cells over-expressing VSV-G-tagged Vinculin. ICC/IF: COS7 cells over-expressing VSV-G tagged vinculin.
  • General notes

    This product was changed from ascites to tissue culture supernatant on 25th October 2016. The following lot(s) is/are from ascites and is still in stock as of 25th October 2016- GR223940,  GR223940. Lot numbers other than GR223940 and GR223940 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

    In infected cells, ab50549 localizes the immature forms of VSV-G in the rough endoplasmic reticulum (RER) and in the cisternae of Golgi complex, as well as mature VSV-G at the cell surface and in the budding virus. The antibody does not stain the secreted form of VSV-G which lacks the membrane and the cytoplasmic domain.


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    pH: 7.4
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Proprietary Purification
  • Purification notes
    Purified from Tissue culture supernatant.
  • Clonality
  • Clone number
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab50549 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 21159872
WB Use a concentration of 0.5 - 1 µg/ml.
IP Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.


  • Relevance
    Vesicular stomatitis virus (VSV), an enveloped RNA virus from the Rhabdoviridae family, is released from the plasma membrane of host cells by a process called budding. The glycoprotein (VSV-g) contains a domain in its extracellular membrane proximal stem that appears to be needed for efficient VSV budding.
  • Alternative names
    • Vesicular stomatitis virus glycoprotein tag antibody
    • VSV epitope tag antibody
    • VSV tag antibody
    • VSV-G epitope tag antibody
    • VSVG antibody
    • vsvg tag antibody
    see all


  • COS7 cells over-expressing VSV-G tagged vinculin were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 5 μg/ml ab50549. The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.

  • Lane 1 : Negative control: without first antibody
    Lane 2 : Anti-VSV-G tag antibody [P5D4] (ab50549) at 0.5 µg/ml

    Lane 1 : Whole extract of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells over-expressing VSV-G-tagged Vinculin
    Lane 2 : Whole extract of HEK-293T cells over-expressing VSV-G-tagged Vinculin

    All lanes : Goat Anti-Mouse IgG-Peroxidase


This product has been referenced in:
  • Malikov V  et al. HIV-1 capsids bind and exploit the kinesin-1 adaptor FEZ1 for inward movement to the nucleus. Nat Commun 6:6660 (2015). WB . Read more (PubMed: 25818806) »
  • Lin AH  et al. Blockade of type I interferon (IFN) production by retroviral replicating vectors and reduced tumor cell responses to IFN likely contribute to tumor selectivity. J Virol 88:10066-77 (2014). Read more (PubMed: 24965455) »

See all 7 Publications for this product

Customer reviews and Q&As

Thank you for contacting us.

Unfortunately we have not tested this antibody with an N-terminal VSVG tagged protein. The epitope for this clone (P5D4) resides within the C-terminal five amino acids (NRLGK) of the 15-amino acid antigenic ...

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Thank you for your enquiry and your interest in our products.

We have a few antibodies which detect VSV-G and are suitable for Western applications. Some of them are unconjugated so it is essential to apply a compatible secondary antibody for...

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