Product nameAnti-VSV-G tag antibody [P5D4]
See all VSV-G tag primary antibodies
DescriptionMouse monoclonal [P5D4] to VSV-G tag
Tested applicationsSuitable for: ICC/IF, WB, IP, ICCmore details
Species reactivityReacts with: Species independent
Synthetic peptide corresponding to C terminal amino acids 497-511 of VSV-G tag conjugated to KLH
- WB: Whole extract of HEK-293T cells over-expressing VSV-G-tagged Vinculin. ICC/IF: COS7 cells over-expressing VSV-G tagged vinculin.
This product was changed from ascites to tissue culture supernatant on 25th October 2016. The following lot(s) is/are from ascites and is still in stock as of 25th October 2016- GR223940, GR223940. Lot numbers other than GR223940 and GR223940 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
In infected cells, ab50549 localizes the immature forms of VSV-G in the rough endoplasmic reticulum (RER) and in the cisternae of Golgi complex, as well as mature VSV-G at the cell surface and in the budding virus. The antibody does not stain the secreted form of VSV-G which lacks the membrane and the cytoplasmic domain.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.4
Preservative: 0.097% Sodium azide
Concentration information loading...
Purification notesPurified from Tissue culture supernatant.
Our Abpromise guarantee covers the use of ab50549 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. PubMed: 21159872|
|WB||Use a concentration of 0.5 - 1 µg/ml.|
|IP||Use at an assay dependent concentration.|
|ICC||Use at an assay dependent concentration.|
RelevanceVesicular stomatitis virus (VSV), an enveloped RNA virus from the Rhabdoviridae family, is released from the plasma membrane of host cells by a process called budding. The glycoprotein (VSV-g) contains a domain in its extracellular membrane proximal stem that appears to be needed for efficient VSV budding.
- Vesicular stomatitis virus glycoprotein tag antibody
- VSV epitope tag antibody
- VSV tag antibody
COS7 cells over-expressing VSV-G tagged vinculin were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 5 μg/ml ab50549. The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Lane 1 : Negative control: without first antibody
Lane 2 : Anti-VSV-G tag antibody [P5D4] (ab50549) at 0.5 µg/ml
Lane 1 : Whole extract of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells over-expressing VSV-G-tagged Vinculin
Lane 2 : Whole extract of HEK-293T cells over-expressing VSV-G-tagged Vinculin
All lanes : Goat Anti-Mouse IgG-Peroxidase
This product has been referenced in:
- Tibbo AJ et al. Methods to Investigate Arrestins in Complex with Phosphodiesterases. Methods Mol Biol 1957:121-137 (2019). Read more (PubMed: 30919351) »
- Bagashev A et al. CD19 Alterations Emerging after CD19-Directed Immunotherapy Cause Retention of the Misfolded Protein in the Endoplasmic Reticulum. Mol Cell Biol 38:N/A (2018). Read more (PubMed: 30104252) »