Overview

  • Product name

    Anti-VSV-G tag antibody [P5D4]
    See all VSV-G tag primary antibodies
  • Description

    Mouse monoclonal [P5D4] to VSV-G tag
  • Host species

    Mouse
  • Tested applications

    Suitable for: ICC/IF, WB, IP, ICCmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Synthetic peptide corresponding to C terminal amino acids 497-511 of VSV-G tag conjugated to KLH

  • Positive control

    • WB: Whole extract of HEK-293T cells over-expressing VSV-G-tagged Vinculin. ICC/IF: COS7 cells over-expressing VSV-G tagged vinculin.
  • General notes

    This product was changed from ascites to tissue culture supernatant on 25th October 2016. The following lot(s) is/are from ascites and is still in stock as of 25th October 2016- GR223940,  GR223940. Lot numbers other than GR223940 and GR223940 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

    In infected cells, ab50549 localizes the immature forms of VSV-G in the rough endoplasmic reticulum (RER) and in the cisternae of Golgi complex, as well as mature VSV-G at the cell surface and in the budding virus. The antibody does not stain the secreted form of VSV-G which lacks the membrane and the cytoplasmic domain.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    pH: 7.4
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Proprietary Purification
  • Purification notes

    Purified from Tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    P5D4
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab50549 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 21159872
WB Use a concentration of 0.5 - 1 µg/ml.
IP Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.

Target

  • Relevance

    Vesicular stomatitis virus (VSV), an enveloped RNA virus from the Rhabdoviridae family, is released from the plasma membrane of host cells by a process called budding. The glycoprotein (VSV-g) contains a domain in its extracellular membrane proximal stem that appears to be needed for efficient VSV budding.
  • Alternative names

    • Vesicular stomatitis virus glycoprotein tag antibody
    • VSV epitope tag antibody
    • VSV tag antibody
    • VSV-G epitope tag antibody
    • VSVG antibody
    • vsvg tag antibody
    see all

Images

  • COS7 cells over-expressing VSV-G tagged vinculin were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 5 μg/ml ab50549. The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.

  • Lane 1 : Negative control: without first antibody
    Lane 2 : Anti-VSV-G tag antibody [P5D4] (ab50549) at 0.5 µg/ml

    Lane 1 : Whole extract of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells over-expressing VSV-G-tagged Vinculin
    Lane 2 : Whole extract of HEK-293T cells over-expressing VSV-G-tagged Vinculin

    Secondary
    All lanes : Goat Anti-Mouse IgG-Peroxidase

References

This product has been referenced in:

  • Tibbo AJ  et al. Methods to Investigate Arrestins in Complex with Phosphodiesterases. Methods Mol Biol 1957:121-137 (2019). Read more (PubMed: 30919351) »
  • Bagashev A  et al. CD19 Alterations Emerging after CD19-Directed Immunotherapy Cause Retention of the Misfolded Protein in the Endoplasmic Reticulum. Mol Cell Biol 38:N/A (2018). Read more (PubMed: 30104252) »
See all 9 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for contacting us.


Unfortunately we have not tested this antibody with an N-terminal VSVG tagged protein. The epitope for this clone (P5D4) resides within the C-terminal five amino acids (NRLGK) of the 15-amino acid antigenic peptide (KRQIYTDIEMNRLGK) so as long as this sequence is still surface exposed on the recombinant protein this antibody should work, however you would need to test this for yourself.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for your enquiry and your interest in our products.

We have a few antibodies which detect VSV-G and are suitable for Western applications. Some of them are unconjugated so it is essential to apply a compatible secondary antibody for detection:

ab1874: https://www.abcam.com/vsv-g-tag-antibody-ab1874.html

ab27026: https://www.abcam.com/vsv-g-tag-antibody-ab27026.html

ab50549: https://www.abcam.com/vsv-g-tag-antibody-p5d4-ab50549.html

Some others are HRP-labelled so can be applied without any secondary antibodies:

ab3556: https://www.abcam.com/vsv-g-tag-antibody-hrp-ab3556.html

ab49610: https://www.abcam.com/vsv-g-tag-antibody-p5d4-hrp-ab49610.html

Currently, we do not sel lpositive control cell lysate for VSV-G. As long as the cells you are planning to use express the tag sequence: YTDIEMNRLGK; these antibodies should work fine.

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