Overview

  • Product name

  • Description

    Rabbit polyclonal to VTI1A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within the N terminal amino acids 2-51 (SSDFEGYEQD FAVLTAEITS KIARVPRLPP DEKKQMVANV EKQLEEAKEL) of human VTI1A (NP_660207).

  • Positive control

    • Human fetal muscle lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab84556 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 25 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function

    V-SNARE that mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane. These interactions are proposed to mediate aspects of the specificity of vesicle trafficking and to promote fusion of the lipid bilayers. Involved in vesicular transport from the late endosomes to the trans-Golgi network. Along with VAMP7, involved in an non-conventional RAB1-dependent traffic route to the cell surface used by KCNIP1 and KCND2. May be involved in increased cytokine secretion associated with cellular senescence.
  • Sequence similarities

    Belongs to the VTI1 family.
  • Cellular localization

    Cytoplasmic vesicle. Golgi apparatus membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • MMDS3 antibody
    • MVti1 antibody
    • SNARE Vti1a beta protein antibody
    • Vesicle transport through interaction with t-SNAREs homolog 1A antibody
    • Vesicle transport through interaction with tSNAREs homolog 1A (yeast) antibody
    • Vesicle transport v-SNARE protein Vti1-like 2 antibody
    • Vti1 rp2 antibody
    • Vti1-rp2 antibody
    • Vti1a antibody
    • VTI1A vesicle transport through interaction with t-SNAREs 1A antibody
    • VTI1A_HUMAN antibody
    • VTI1RP2 antibody
    see all

Images

  • Anti-VTI1A antibody (ab84556) at 1 µg/ml (in 5% skim milk / PBS buffer) + human fetal muscle lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 25 kDa
    Observed band size: 29 kDa
    why is the actual band size different from the predicted?



    Gel concentration: 12%

References

ab84556 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab84556.
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