Overview

  • Product name
    Anti-VTI1B antibody [EPR15506(B)]
    See all VTI1B primary antibodies
  • Description
    Rabbit monoclonal [EPR15506(B)] to VTI1B
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human VTI1B aa 1-100. The exact sequence is proprietary.
    Database link: Q9UEU0

  • Positive control
    • MCF7, HeLa, Human fetal lung and fetal liver lysates; Human ovarian carcinoma tissue; MCF7 and HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR15506(B)
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab184170 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100.
WB 1/1000 - 1/10000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).
ICC/IF 1/50.
Flow Cyt 1/170.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    V-SNARE that mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane. These interactions are proposed to mediate aspects of the specificity of vesicle trafficking and to promote fusion of the lipid bilayers. May be concerned with increased secretion of cytokines associated with cellular senescence.
  • Tissue specificity
    Expressed in all tissues examined.
  • Sequence similarities
    Belongs to the VTI1 family.
  • Cellular localization
    Endomembrane system. Cytoplasmic granule.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • HGNC:17793 antibody
    • V SNARE antibody
    • v-SNARE antibody
    • Vesicle associated soluble NSF attachment protein receptor antibody
    • Vesicle transport through interaction with t SNAREs 1B antibody
    • Vesicle transport through interaction with t SNAREs homolog 1B (yeast) antibody
    • Vesicle transport through interaction with t-SNAREs homolog 1B antibody
    • Vesicle transport v-SNARE protein Vti1-like 1 antibody
    • VTI1 antibody
    • VTI1 like antibody
    • Vti1 rp1 antibody
    • VTI1-LIKE antibody
    • Vti1-rp1 antibody
    • VTI1B antibody
    • VTI1B_HUMAN antibody
    • VTI1L antibody
    • VTI1L1 antibody
    • VTI2 antibody
    see all

Images

  • Western blot - Anti-VTI1B antibody [EPR15506(B)] (ab184170)
    Western blot - Anti-VTI1B antibody [EPR15506(B)] (ab184170)
    All lanes : Anti-VTI1B antibody [EPR15506(B)] (ab184170) at 1/2000 dilution

    Lane 1 : MCF7 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Human fetal liver lysate
    Lane 4 : Human fetal lung lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 27 kDa

  • Western blot - Anti-VTI1B antibody [EPR15506(B)] (ab184170)
    Western blot - Anti-VTI1B antibody [EPR15506(B)] (ab184170)
    Anti-VTI1B antibody [EPR15506(B)] (ab184170) at 1/10000 dilution + MCF7 cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 27 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VTI1B antibody [EPR15506(B)] (ab184170)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VTI1B antibody [EPR15506(B)] (ab184170)

    Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma labeling VTI1B with ab184170 at 1:100 diluton and HRP polymer.  Counterstained with Hematoxylin.

  • Immunocytochemistry/ Immunofluorescence - Anti-VTI1B antibody [EPR15506(B)] (ab184170)
    Immunocytochemistry/ Immunofluorescence - Anti-VTI1B antibody [EPR15506(B)] (ab184170)

    Immunofluorescent analysis of MCF7 cells (paraformaldehyde-fixed, 4%) labeling VTI1B with ab184170 at 1/50 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

  • Flow Cytometry - Anti-VTI1B antibody [EPR15506(B)] (ab184170)
    Flow Cytometry - Anti-VTI1B antibody [EPR15506(B)] (ab184170)

    Flow cytometric analysis of Hela cells (2% paraformaldehyde-fixed) labeling VTI1B with ab184170 at 1/170 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

References

This product has been referenced in:
  • Lesteberg K  et al. Recycling endosomes in human cytotoxic T lymphocytes constitute an auxiliary intracellular trafficking pathway for newly synthesized perforin. Immunol Res 65:1031-1045 (2017). Flow Cyt ; Human . Read more (PubMed: 28822075) »

See 1 Publication for this product

Publishing research using ab184170? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Submit a review Submit a question

There are currently no Customer reviews or Questions for ab184170.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up