Recombinant Anti-VTI1B antibody [EPR15506(B)] (ab184170)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15506(B)] to VTI1B
- Suitable for: IHC-P, WB, ICC/IF, Flow Cyt (Intra)
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-VTI1B antibody [EPR15506(B)]
See all VTI1B primary antibodies -
Description
Rabbit monoclonal [EPR15506(B)] to VTI1B -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IF, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: MCF7 and HeLa cell lysates; Human fetal lung and fetal liver lysates. IHC-P: Human ovarian carcinoma tissue. ICC/IF: MCF7 cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15506(B) -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Conjugation kits
-
Isotype control
-
KO cell lines
-
KO cell lysates
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab184170 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
WB |
1/1000 - 1/10000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).
|
|
ICC/IF |
1/50.
|
|
Flow Cyt (Intra) |
1/170.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Notes |
---|
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
1/1000 - 1/10000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa). |
ICC/IF
1/50. |
Flow Cyt (Intra)
1/170. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
-
Function
V-SNARE that mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane. These interactions are proposed to mediate aspects of the specificity of vesicle trafficking and to promote fusion of the lipid bilayers. May be concerned with increased secretion of cytokines associated with cellular senescence. -
Tissue specificity
Expressed in all tissues examined. -
Sequence similarities
Belongs to the VTI1 family. -
Cellular localization
Endomembrane system. Cytoplasmic granule. - Information by UniProt
-
Database links
- Entrez Gene: 10490 Human
- Entrez Gene: 53612 Mouse
- GenBank: BC003142 Human
- Omim: 603207 Human
- SwissProt: Q9UEU0 Human
- SwissProt: O88384 Mouse
- SwissProt: P58200 Rat
- Unigene: 728787 Human
see all -
Alternative names
- HGNC:17793 antibody
- V SNARE antibody
- v-SNARE antibody
see all
Images
-
All lanes : Anti-VTI1B antibody [EPR15506(B)] (ab184170) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : VTI1B knockout HeLa cell lysate
Lane 3 : MCF7 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 27 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?Lanes 1-3: Merged signal (red and green). Green - ab184170 observed at 28 kDa. Red - loading control ab8245 observed at 36 kDa.
ab184170 Anti-VTI1B antibody [EPR15506(B)] was shown to specifically react with VTI1B in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265483 (knockout cell lysate ab257797) was used. Wild-type and VTI1B knockout samples were subjected to SDS-PAGE. ab184170 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
-
Intracellular flow cytometric analysis of Hela cells (2% paraformaldehyde-fixed) labeling VTI1B with ab184170 at 1/170 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
-
All lanes : Anti-VTI1B antibody [EPR15506(B)] (ab184170) at 1/2000 dilution
Lane 1 : MCF7 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Human fetal liver lysate
Lane 4 : Human fetal lung lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 27 kDa -
Anti-VTI1B antibody [EPR15506(B)] (ab184170) at 1/10000 dilution + MCF7 cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 27 kDa -
Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma labeling VTI1B with ab184170 at 1:100 diluton and HRP polymer. Counterstained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
-
Immunofluorescent analysis of MCF7 cells (paraformaldehyde-fixed, 4%) labeling VTI1B with ab184170 at 1/50 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (4)
ab184170 has been referenced in 4 publications.
- Coukos R et al. An engineered transcriptional reporter of protein localization identifies regulators of mitochondrial and ER membrane protein trafficking in high-throughput CRISPRi screens. Elife 10:N/A (2021). PubMed: 34414886
- Margiotta A et al. Invariant chain regulates endosomal fusion and maturation through an interaction with the SNARE Vti1b. J Cell Sci 133:N/A (2020). PubMed: 32907852
- Abed M et al. The Gag protein PEG10 binds to RNA and regulates trophoblast stem cell lineage specification. PLoS One 14:e0214110 (2019). PubMed: 30951545
- Lesteberg K et al. Recycling endosomes in human cytotoxic T lymphocytes constitute an auxiliary intracellular trafficking pathway for newly synthesized perforin. Immunol Res 65:1031-1045 (2017). Flow Cyt ; Human . PubMed: 28822075