Key features and details
- Rabbit polyclonal to VT/Stx
- Suitable for: WB, IP, ELISA
- Reacts with: Species independent
- Isotype: IgG
Product nameAnti-VT/Stx antibody
DescriptionRabbit polyclonal to VT/Stx
VT1 and VT2 of E. coli VTEC strain and Shiga toxin of Shigella dysenteriae 1.
Tested applicationsSuitable for: WB, IP, ELISAmore details
Species reactivityReacts with: Species independent
Full length native protein (purified) corresponding to VT/Stx. Initial immunization by VT1 toxoid and booster by VT1 toxin.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6
Preservative: 0.09% Sodium azide
Concentration information loading...
Our Abpromise guarantee covers the use of ab188542 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Predicted molecular weight: 36 kDa.|
|IP||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration.|
Immunoprecipitation of VT1 from culture medium of VTEC with ab188542. Arrows shows subunit A and subunit B of VT1. Heavy chain and Light chain indicate those of IgG.
All lanes : Anti-VT/Stx antibody (ab188542) at 1/2000 dilution
Lane 1 : culture medium of VTEC
Lane 2 : crude extracts of VTEC cells
Predicted band size: 36 kDa
All lanes : Anti-VT/Stx antibody (ab188542)
Lane 1 : Culture medium of E. coli O157:H7
Lane 2 : Purified VT1
Lane 3 : Purified VT2
Predicted band size: 36 kDa
ab188542 has not yet been referenced specifically in any publications.