Key features and details
- Rabbit polyclonal to WAC - C-terminal
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-WAC antibody - C-terminal
See all WAC primary antibodies
DescriptionRabbit polyclonal to WAC - C-terminal
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
Recombinant fragment within Human WAC (C terminal). The exact sequence is proprietary.
Database link: Q9BTA9
- WB: HepG2 whole cell extract. IHC-P: Mouse testis tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229574 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 71 kDa.|
FunctionActs as a linker between gene transcription and histone H2B monoubiquitination at 'Lys-120' (H2BK120ub1). Interacts with the RNA polymerase II transcriptional machinery via its WW domain and with RNF20-RNF40 via its coiled coil region, thereby linking and regulating H2BK120ub1 and gene transcription. Regulates the cell-cycle checkpoint activation in response to DNA damage.
Sequence similaritiesContains 1 WW domain.
modificationsPhosphorylated on tyrosine residues.
Cellular localizationNucleus speckle. Nucleus. In distinct nuclear speckles. Colocalizes with pre-mRNA processing complexes.
- Information by UniProt
- 1110067P07Rik antibody
- BA48B24 antibody
- BA48B24.1 antibody
Paraffin-embedded mouse testis tissue stained for WAC using ab229574 at 1/1000 dilution in immunohistochemical analysis
Anti-WAC antibody - C-terminal (ab229574) at 1/500 dilution + HepG2 (human liver hepatocellular carcinoma cell line) while cell extract at 30 µg
Predicted band size: 71 kDa
7.5% SDS PAGE gel.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229574 has not yet been referenced specifically in any publications.