Anti-WAPL/FOE antibody (ab70741)
Key features and details
- Rabbit polyclonal to WAPL/FOE
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
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Overview
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Product name
Anti-WAPL/FOE antibody
See all WAPL/FOE primary antibodies -
Description
Rabbit polyclonal to WAPL/FOE -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Rhesus monkey, Gorilla, Platypus -
Immunogen
Synthetic peptide corresponding to Human WAPL/FOE aa 1125-1175.
Database link: Q7Z5K2 -
Positive control
- HeLa nuclear extract.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab70741 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/2500 - 1/10000. Detects a band of approximately 175 kDa (predicted molecular weight: 133 kDa).
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Notes |
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WB
1/2500 - 1/10000. Detects a band of approximately 175 kDa (predicted molecular weight: 133 kDa). |
Target
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Function
Regulator of sister chromatid cohesion in mitosis which negatively regulates cohesin association with chromatin. Involved in both sister chromatid cohesion during interphase and sister-chromatid resolution during early stages of mitosis. Couples DNA replication to sister chromatid cohesion. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair. -
Tissue specificity
Isoform 1 is highly expressed in uterine cervix tumor. Isoform 2 is widely expressed with a high level in skeletal muscle and heart. -
Sequence similarities
Contains 1 WAPL domain. -
Cellular localization
Nucleus and Nucleus. Chromosome. Cytoplasm. Associates with chromatin through the cohesin complex during interphase. Released in the cytoplasm from nuclear envelope breakdown until anaphase, it reaccumulates in nucleus at telophase. - Information by UniProt
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Database links
- Entrez Gene: 23063 Human
- Entrez Gene: 218914 Mouse
- Entrez Gene: 290577 Rat
- Omim: 610754 Human
- SwissProt: Q7Z5K2 Human
- SwissProt: Q65Z40 Mouse
- Unigene: 203099 Human
- Unigene: 714876 Human
see all -
Alternative names
- FOE antibody
- Friend of EBNA2 (Epstein Barr virus nuclear protein 2) antibody
- Friend of EBNA2 protein antibody
see all
Images
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All lanes : Anti-WAPL/FOE antibody (ab70741) at 0.2 µg/ml
Lane 1 : HeLa nuclear extract at 5 µg
Lane 2 : HeLa nuclear extract at 20 µg
Predicted band size: 133 kDa
Observed band size: 175 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes -
All lanes : Anti-WAPL/FOE antibody (ab70741) at 1/2000 dilution
Lane 1 : Human U2OS cells
Lane 2 : Human U2OS cells treated with siRNA #1 against WAPL/FOE
Lane 3 : Human U2OS cells treated with siRNA #2 against WAPL/FOE
Lysates/proteins at 90 µg per lane.
Secondary
All lanes : Immunopure Pierce anti-rabbit HRP conjugated at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 133 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab70741 has been referenced in 7 publications.
- Shiimori M et al. Suv4-20h2 protects against influenza virus infection by suppression of chromatin loop formation. iScience 24:102660 (2021). PubMed: 34169237
- Kriz AJ et al. Balancing cohesin eviction and retention prevents aberrant chromosomal interactions, Polycomb-mediated repression, and X-inactivation. Mol Cell 81:1970-1987.e9 (2021). PubMed: 33725485
- Gu B et al. Opposing Effects of Cohesin and Transcription on CTCF Organization Revealed by Super-resolution Imaging. Mol Cell 80:699-711.e7 (2020). PubMed: 33091336
- Perea-Resa C et al. Cohesin Removal Reprograms Gene Expression upon Mitotic Entry. Mol Cell 78:127-140.e7 (2020). PubMed: 32035037
- Meisenberg C et al. Repression of Transcription at DNA Breaks Requires Cohesin throughout Interphase and Prevents Genome Instability. Mol Cell 73:212-223.e7 (2019). PubMed: 30554942
- Eykelenboom JK et al. Live imaging of marked chromosome regions reveals their dynamic resolution and compaction in mitosis. J Cell Biol 218:1531-1552 (2019). PubMed: 30858191
- Stelloh C et al. The cohesin-associated protein Wapal is required for proper Polycomb-mediated gene silencing. Epigenetics Chromatin 9:14 (2016). PubMed: 27087855