Key features and details
- Mouse polyclonal to WAPL/FOE
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-WAPL/FOE antibody
See all WAPL/FOE primary antibodies
DescriptionMouse polyclonal to WAPL/FOE
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Full length protein corresponding to Human WAPL/FOE aa 1-402.
MDLDRASLDLMIRLLELEQDASSAKLLNEKDMNKIKEKIRRLCETVHNKH LDLENITTGHLAMETLLSLTSKRAGDWFKEELRLLGGLDHIVDKVKECVD HLSRDEDEEKLVASLWGAERCLRVLESVTVHNPENQSYLIAYKDSQLIVS SAKALQHCEELIQQYNRAEDSICLADSKPLPHQNVTNHVGKAVEDCMRAI IGVLLNLTNDNEWGSTKTGEQDGLIGTALNCVLQVPKYLPQEQRFDIRVL LFLERERAAQLAESKTDELIKDAPTTQHDKSGEWQETSGEIQWVSTEKTD GTEEKHKKEEEDEELDLNKALQHAGKHMEDCIVASYTALLLGCLCQESPI NVTTVREYLPEGDFSIMTEMLKKFLSFMNLTCAVGTTGQKSISRVIEYLE HC
- WB: 293T cells transfected with WAPL/FOE. IF: HeLa cells.
This product was previously labelled as WAPL
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.4
Constituent: 99% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab168612 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 133 kDa.|
|ICC/IF||Use a concentration of 10 µg/ml.|
FunctionRegulator of sister chromatid cohesion in mitosis which negatively regulates cohesin association with chromatin. Involved in both sister chromatid cohesion during interphase and sister-chromatid resolution during early stages of mitosis. Couples DNA replication to sister chromatid cohesion. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair.
Tissue specificityIsoform 1 is highly expressed in uterine cervix tumor. Isoform 2 is widely expressed with a high level in skeletal muscle and heart.
Sequence similaritiesContains 1 WAPL domain.
Cellular localizationNucleus and Nucleus. Chromosome. Cytoplasm. Associates with chromatin through the cohesin complex during interphase. Released in the cytoplasm from nuclear envelope breakdown until anaphase, it reaccumulates in nucleus at telophase.
- Information by UniProt
- FOE antibody
- Friend of EBNA2 (Epstein Barr virus nuclear protein 2) antibody
- Friend of EBNA2 protein antibody
All lanes : Anti-WAPL/FOE antibody (ab168612) at 1 µg/ml
Lane 1 : Lysate of 293T cells transfected with WAPL/FOE (44.22 KDa)
Lane 2 : Lysate of untransfected 293T cells
Lysates/proteins at 15 µl per lane.
Predicted band size: 133 kDa
Immunofluorescence analysis of HeLa cells labeling WAPAL with ab168612 at 10 µg/ml.
ab168612 has not yet been referenced specifically in any publications.