Key features and details
- Rabbit polyclonal to WAPL/FOE
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-WAPL/FOE antibody
See all WAPL/FOE primary antibodies
DescriptionRabbit polyclonal to WAPL/FOE
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Rhesus monkey, Gorilla, Platypus
Synthetic peptide corresponding to Human WAPL/FOE aa 1125-1175.
Database link: Q7Z5K2
- HeLa nuclear extract.
This product was previously labelled as WAPL
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab70741 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2500 - 1/10000. Detects a band of approximately 175 kDa (predicted molecular weight: 133 kDa).|
FunctionRegulator of sister chromatid cohesion in mitosis which negatively regulates cohesin association with chromatin. Involved in both sister chromatid cohesion during interphase and sister-chromatid resolution during early stages of mitosis. Couples DNA replication to sister chromatid cohesion. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair.
Tissue specificityIsoform 1 is highly expressed in uterine cervix tumor. Isoform 2 is widely expressed with a high level in skeletal muscle and heart.
Sequence similaritiesContains 1 WAPL domain.
Cellular localizationNucleus and Nucleus. Chromosome. Cytoplasm. Associates with chromatin through the cohesin complex during interphase. Released in the cytoplasm from nuclear envelope breakdown until anaphase, it reaccumulates in nucleus at telophase.
- Information by UniProt
- FOE antibody
- Friend of EBNA2 (Epstein Barr virus nuclear protein 2) antibody
- Friend of EBNA2 protein antibody
All lanes : Anti-WAPL/FOE antibody (ab70741) at 0.2 µg/ml
Lane 1 : HeLa nuclear extract at 5 µg
Lane 2 : HeLa nuclear extract at 20 µg
Predicted band size: 133 kDa
Observed band size: 175 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes
All lanes : Anti-WAPL/FOE antibody (ab70741) at 1/2000 dilution
Lane 1 : Human U2OS cells
Lane 2 : Human U2OS cells treated with siRNA #1 against WAPL/FOE
Lane 3 : Human U2OS cells treated with siRNA #2 against WAPL/FOE
Lysates/proteins at 90 µg per lane.
All lanes : Immunopure Pierce anti-rabbit HRP conjugated at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 133 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
ab70741 has been referenced in 3 publications.
- Meisenberg C et al. Repression of Transcription at DNA Breaks Requires Cohesin throughout Interphase and Prevents Genome Instability. Mol Cell 73:212-223.e7 (2019). PubMed: 30554942
- Eykelenboom JK et al. Live imaging of marked chromosome regions reveals their dynamic resolution and compaction in mitosis. J Cell Biol 218:1531-1552 (2019). PubMed: 30858191
- Stelloh C et al. The cohesin-associated protein Wapal is required for proper Polycomb-mediated gene silencing. Epigenetics Chromatin 9:14 (2016). PubMed: 27087855