Overview

  • Product name

    Anti-WAPL/FOE antibody [EPR3407(2)]
    See all WAPL/FOE primary antibodies
  • Description

    Rabbit monoclonal [EPR3407(2)] to WAPL/FOE
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, ICC/IFmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human WAPL/FOE aa 50-150. The exact sequence is proprietary.

  • Positive control

    • HeLa, 293T, and Jurkat cell lysates. HeLa cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

     This product was previously labelled as WAPL

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab109537 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50000 - 1/200000. Predicted molecular weight: 133 kDa.
IP 1/10 - 1/100.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.
  • Target

    • Function

      Regulator of sister chromatid cohesion in mitosis which negatively regulates cohesin association with chromatin. Involved in both sister chromatid cohesion during interphase and sister-chromatid resolution during early stages of mitosis. Couples DNA replication to sister chromatid cohesion. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair.
    • Tissue specificity

      Isoform 1 is highly expressed in uterine cervix tumor. Isoform 2 is widely expressed with a high level in skeletal muscle and heart.
    • Sequence similarities

      Contains 1 WAPL domain.
    • Cellular localization

      Nucleus and Nucleus. Chromosome. Cytoplasm. Associates with chromatin through the cohesin complex during interphase. Released in the cytoplasm from nuclear envelope breakdown until anaphase, it reaccumulates in nucleus at telophase.
    • Information by UniProt
    • Database links

    • Alternative names

      • FOE antibody
      • Friend of EBNA2 (Epstein Barr virus nuclear protein 2) antibody
      • Friend of EBNA2 protein antibody
      • KIAA0261 antibody
      • OTTHUMP00000020004 antibody
      • WAPAL antibody
      • WAPL antibody
      • WAPL_HUMAN antibody
      • Wings apart like homolog antibody
      • Wings apart like homolog (Drosophila) antibody
      • Wings apart-like protein homolog antibody
      see all

    Images

    • All lanes : Anti-WAPL/FOE antibody [EPR3407(2)] (ab109537) at 1/50000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP-labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 133 kDa

    • ab109537 at 1/100 dilution staining WAPL/FOE in HeLa (Human epithelial cell line from cervix adenocarcinoma) cells by Immunofluorescence.

    • All lanes : Anti-WAPL/FOE antibody [EPR3407(2)] (ab109537) at 1/10000 dilution

      Lane 1 : Human U2OS cells treated with siRNA control.
      Lane 2 : Human U2OS cells treated with siRNA #1 against WAPL/FOE
      Lane 3 : Human U2OS cells treated with siRNA #2 against WAPL/FOE

      Lysates/proteins at 90 µg per lane.

      Secondary
      Lane 1 : Immunopure Pierce anti-rabbit HRP conjugated at 1/10000 dilution
      Lanes 2-3 : Immunopure Pierce anti-rabbit HRP conjugated at 1/10000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 133 kDa
      Observed band size: 150 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 120 kDa (possible cross reactivity), 130 kDa (possible cross reactivity), 145 kDa (possible cross reactivity)

      See Abreview

    References

    This product has been referenced in:

    • Kanke M  et al. Cohesin acetylation and Wapl-Pds5 oppositely regulate translocation of cohesin along DNA. EMBO J 35:2686-2698 (2016). Read more (PubMed: 27872142) »
    See 1 Publication for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Human osteosarcoma U2OS cells)
    Gel Running Conditions
    Reduced Denaturing (8% SDS-PAGE)
    Loading amount
    90 µg
    Specification
    Human osteosarcoma U2OS cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Oct 10 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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