Recombinant Anti-WAPL/FOE antibody [EPR3407(2)] (ab109537)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-WAPL/FOE antibody [EPR3407(2)]
See all WAPL/FOE primary antibodies -
Description
Rabbit monoclonal [EPR3407(2)] to WAPL/FOE -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ICC/IFmore details
Unsuitable for: Flow Cyt or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human WAPL/FOE aa 50-150. The exact sequence is proprietary.
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Positive control
- HeLa, 293T, and Jurkat cell lysates. HeLa cells.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as WAPL
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR3407(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab109537 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/50000 - 1/200000. Predicted molecular weight: 133 kDa. | |
| IP | 1/10 - 1/100. | |
| ICC/IF | 1/100 - 1/250. |
Target
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Function
Regulator of sister chromatid cohesion in mitosis which negatively regulates cohesin association with chromatin. Involved in both sister chromatid cohesion during interphase and sister-chromatid resolution during early stages of mitosis. Couples DNA replication to sister chromatid cohesion. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair. -
Tissue specificity
Isoform 1 is highly expressed in uterine cervix tumor. Isoform 2 is widely expressed with a high level in skeletal muscle and heart. -
Sequence similarities
Contains 1 WAPL domain. -
Cellular localization
Nucleus and Nucleus. Chromosome. Cytoplasm. Associates with chromatin through the cohesin complex during interphase. Released in the cytoplasm from nuclear envelope breakdown until anaphase, it reaccumulates in nucleus at telophase. - Information by UniProt
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Database links
- Entrez Gene: 23063 Human
- Omim: 610754 Human
- SwissProt: Q7Z5K2 Human
- Unigene: 203099 Human
- Unigene: 714876 Human
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Alternative names
- FOE antibody
- Friend of EBNA2 (Epstein Barr virus nuclear protein 2) antibody
- Friend of EBNA2 protein antibody
see all
Images
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![Western blot - Anti-WAPL/FOE antibody [EPR3407(2)] (ab109537)](https://www.abcam.com/ps/products/109/ab109537/Images/ab109537-122482-anti-wapl-antibody-epr34072-western-blot.jpg)
Western blot - Anti-WAPL/FOE antibody [EPR3407(2)] (ab109537)All lanes : Anti-WAPL/FOE antibody [EPR3407(2)] (ab109537) at 1/50000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 133 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-WAPL/FOE antibody [EPR3407(2)] (ab109537)](https://www.abcam.com/ps/products/109/ab109537/Images/ab109537-122481-anti-wapl-antibody-epr34072-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-WAPL/FOE antibody [EPR3407(2)] (ab109537)ab109537 at 1/100 dilution staining WAPL/FOE in HeLa (Human epithelial cell line from cervix adenocarcinoma) cells by Immunofluorescence.
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![Western blot - Anti-WAPL/FOE antibody [EPR3407(2)] (ab109537)](https://www.abcam.com/ps/products/109/ab109537/Images/ab109537-122480-anti-wapl-antibody-epr34072-western-blot.jpg)
Western blot - Anti-WAPL/FOE antibody [EPR3407(2)] (ab109537)All lanes : Anti-WAPL/FOE antibody [EPR3407(2)] (ab109537) at 1/10000 dilution
Lane 1 : Human U2OS cells treated with siRNA control.
Lane 2 : Human U2OS cells treated with siRNA #1 against WAPL/FOE
Lane 3 : Human U2OS cells treated with siRNA #2 against WAPL/FOE
Lysates/proteins at 90 µg per lane.
Secondary
Lane 1 : Immunopure Pierce anti-rabbit HRP conjugated at 1/10000 dilution
Lanes 2-3 : Immunopure Pierce anti-rabbit HRP conjugated at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 133 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Additional bands at: 120 kDa (possible cross reactivity), 130 kDa (possible cross reactivity), 145 kDa (possible cross reactivity)
Protocols
Datasheets and documents
References
This product has been referenced in:
- Kanke M et al. Cohesin acetylation and Wapl-Pds5 oppositely regulate translocation of cohesin along DNA. EMBO J 35:2686-2698 (2016). Read more (PubMed: 27872142) »
